Figure 6

Lysotracker and Lamp 2 labeling of wild-type and homozygous Cb Cln3^Δex7/8 lysosomes Lysosomal labeling of wild-type and homozygous CbCln3^Δex7/8 precursor cells with lysotracker and Lamp 2 antibody is shown. Lysotracker dye (top panels) labeled large, perinuclear-clustered lysosomes and scattered lysosomes in the periphery of wild-type cells (CbCln3^+/+). Lysotracker stain was dramatically reduced in homozygous mutant cells (CbCln3^{Δex7/8/Δex7/8}), with smaller labeled vesicles and less apparent perinuclear clustering. Lamp 2 (bottom panels) immunostaining also showed reduced signal intensity with less perinuclear clustering in homozygous CbCln3^Δex7/8 cells, although the effect was somewhat less dramatic than that observed with Lysotracker dye. Wild-type and homozygous CbCln3^Δex7/8 confocal images were captured with identical exposure settings. 60 × magnification.