Table 1 Antibodies used
Marker | Species | Working dilution | Vendor or productor |
|---|---|---|---|
Proliferation | |||
BrdU | Mouse IgG | 1:500 | Novo Castra (Newcastle, UK) |
BrdU | Rat IgG | 1:500 | Accurate Chemicals (New York, USA) |
Magnocellular neurons | |||
OT | Rabbit IgG | 1:2000 | Produced by G. Alonso |
VP | Rabbit IgG | 1:2000 | Produced by G. Alonso |
Astrocytes | |||
GFAP | Rabbit IgG | 1:5000 | Dakko (Glostrup, Denmark) |
Microglia and endothelial cells | |||
Lectin from bandeirae | 1:2000 | Sigma | |
Simplifolia (IsoB4) | |||
Oligodendrocyte progenitors | |||
NG2 | Rabbit IgG | 1:500 | Chemicon (Temecula, USA) |
Vessels | |||
Rat IgG-Alexa 488 conjugated | Goat IgG | 1:2000 | Molecular Probes (Eugene, USA) |
EBA | Mouse IGM | 1:5000 | Sternberger Monoclonals (Lutherville, USA) |
Nestin | Mouse IgG | 1:100 | Hybridoma Bank (Iowa city, USA) |
Vimentin | Mouse IgG | 1:5000 | Sigma |