Figure 1From: The recombination activation gene 1 (Rag1) is expressed in a subset of zebrafish olfactory neurons but is not essential for axon targeting or amino acid detectionImmunofluorescent labelling of RAG1. (A) Isolated zebrafish thymocytes, labelled with the antibody to RAG1 (in red) and DAPI (blue). (B) After pre-absorption with the peptide used for immunization, no labelling was detected. (C-E) Double-label of olfactory epithelial cells isolated from a Rag1:GFP transgenic fish. RAG1 protein (C, D; red) is present in the GFP-positive (C, E; green) neurons (arrow and arrowhead). Neurons with high (arrow) or low (arrowhead) GFP levels contain RAG1. DAPI (C; blue) is used to stain nuclei. Bar = 5 μm.Back to article page