Figure 5

Denervation of the outer molecular layer of the dentate gyrus after entorhinal cortex lesion: A and B: Synaptophysin immunoreactivity in the molecular layer of the dentate gyrus two days after ECL A: ipsilateral dentate, B: contralateral dentate. Note diminished immunostaining and loss of tri-laminar pattern in the OML after ECL. C and D: GFAP immunoreactivity in the molecular layer of the dentate gyrus seven days after ECL. Note increased astroglial expression of GFAP observed after deafferentation of the ipsilateral (C), but not contralateral (D), OML. E: Synaptophysin levels in extracts from fresh micro-dissected (needle punch, see Fig. 1) dentate gyrus tissue as measured by ELISA. Synaptophysin immunoreactivity was reduced two and seven days post-lesion. The ipsilateral side was not different from contralateral dentate thirty days after lesion. oml = outer molecular layer, mml = middle molecular layer, iml = inner molecular layer, and gcl = granule cell layer.