Figure 14

Co-immunoprecipitation (IP) experiments showing interaction of B3 with Rin. Total lysates and IPs were analyzed by Western blot (WB) using antibodies as indicated in the figures. (A) COS-7 cells were co-transfected with pIRES/B3 encoding non-tagged full-length human B3 and pFLAG/Rin encoding FLAG-tagged full-length human Rin. Cells co-transfected with pIRES/B3 and pFLAG vector without insert served as negative control. IP was performed using anti-FLAG antibodies. (B) COS-7 cells were co-transfected with pFLAG/B2 encoding FLAG-tagged full-length human plexin B2 and pFLAG/Rin encoding FLAG-tagged full-length human Rin. Cells co-transfected with pFLAG/B2 and pFLAG vector without insert served as negative control. IP was performed using anti-Rin antibodies. Bands marked with * represent antibodies precipitated by protein-A-agarose.