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Figure 1 | BMC Neuroscience

Figure 1

From: Epidermal growth factor (EGF) withdrawal masks gene expression differences in the study of pituitary adenylate cyclase-activating polypeptide (PACAP) activation of primary neural stem cell proliferation

Figure 1

Neurosphere culturing and experimental design. Neural stem/progenitor cells were isolated from the lateral ventricle wall region of brains from a pool of mice and grown as neurospheres. RNA was isolated from different treatments as indicated and used for subsequent microarray hybridisations. a and b indicate biological replicates. Each arrow represents the number of hybridisations, arrowhead represents labelling with Cy5 and arrow tail represents labelling with Cy3. Groups 1, 2 and 3 indicate hybridisations grouped in the data analysis, to optimise the variance estimates for each gene. NS = neurosphere control, PACAP = pituitary adenylate cyclase-activating polypeptide treated samples, Prol Cont = proliferation control (transmembrane receptor agonist treated samples), Diff Cont = differentiation control (fetal calf serum treated, and solid support plated samples).

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