Figure 1

Immunoblot using the Kv1.1 N-terminal antibody on brain lysate from wild type, Kv1.1-null and mceph/mceph. A. Lysate from wild type (+/+), Kv1.1 null (-/-) and mceph/mceph (m/m) brains were loaded on SDS-PAGE and immunoblotted with the polyclonal Kv1.1 N-terminal antibody. In wild type lysate a strong band was detected at 86 kDa, corresponding to Kv1.1. The same band was seen in the Kv1.1 null and mceph/mceph lysates but at a lower intensity. The 86 kDa bands in Kv1.1 null and mceph/mceph lysates are due to antibody cross reactivity since neither Kv1.1 null nor mceph/mceph mice have any full-length Kv1.1 protein. B. The polyclonal Kv1.1 N-terminal antibody was preincubated with the peptide used for immunization. Lysate from wild type (+/+) and mceph/mceph (m/m) brains were loaded on SDS-PAGE and immunoblotted with the Kv1.1 N-terminal antibody without or after preincubation. The preincubation completely blocked the signal. C. A longer exposure of the immunoblot in panel A. In mceph/mceph brain lysate there was a unique band at approximately 30 kDa, which corresponds to the calculated weight of MCEPH (arrow).