Figure 2

A, Cumulative histograms showing average (error bars indicate SEM) fraction of clusters in cells as a function of cluster size. The data analyzed were from the neurons described in Figure 1. [Asterisks indicate a statistically significant change from control for 1 or more long Homer protein groups.] B, An illustration of the particle analysis for a Homer 2b co-expressing cell. Left shows the image of the region of interest for this cell. Right shows the areas exceeding 1.5× the average fluorescence of the region of interest. 'Clusters' were defined as contiguous regions above this threshold. Two examples of clusters of various sizes are shown. Differences were seen in cluster size ranging from 10 to 50 pixels². Asterisks indicate bins at which all long Homer groups differed from control and + Homer 1a cells. Scale bar is 5 μm.