Figure 3

Effects of SL327 on the induction and expression of locomotor sensitization by repeated administration of D-amph. A) Locomotor responses were measured during 5 consecutive days after administration of D-amph (2 mg/kg, i.p.) 30 min after pre-treatment with vehicle (Veh) or SL327 (30 mg/kg), as indicated (10 mice per group). After 10 days of withdrawal (day 15), the Veh/D-amph and SL327/D-amph groups received an additional challenge with Veh/D-amph or SL327/D-amph, respectively. The following day (day 16, Additional Challenge), mice treated previously with Veh/D-amph were challenged with D-amph in the presence of SL327 whereas those treated previously with SL327/D-amph received an injection of D-amph alone. B) Locomotor responses measured during 5 consecutive days after administration of saline 30 min after pre-treatment with vehicle (Veh) or SL327 (30 mg/kg), as indicated (10 mice per group). After 10 days of withdrawal (day 15), the Veh/saline and SL327/saline groups received an additional treatment with Veh/D-amph. Data were analyzed with a mixed factor ANOVA (repeated measure over time): for saline group (Days 1–5: effect of time: F_(4,90) = 2.15, NS; effect of SL327: F_(1,90) = 1.07, NS); for D-amph group (Days 1–15: effect of time: F_(6,126) = 13.48, P < 0.01; effect of SL327: F_(1,126) = 26.21, P < 0.01). Bonferroni test: * P < 0.01 compared to day 1, ° P < 0.01 Veh/D-amph vs SL327/D-amph.