Figure 2
From: Detection of human and rodent 5-HT3Breceptor subunits by anti-peptide polyclonal antibodies
Immunolabelling by pAb77 of 5-HT 3A and 5-HT 3B receptor subunits expressed in HEK293 cells. Confocal images of HEK293 cells fixed with 4% paraformaldehyde probed with immune sera and visualised by indirect immunofluorescence (FITC). (a) transfection of h5-HT3B probed with pre-immune sera (1:1000); (b) transient transfection of h5-HT3B probed with pAb77 (1:1000); (c) transient transfection of h5-HT3B and m5-HT3A probed with pAb77 (1:1000); (d) permeabilised cells (both panels) transiently transfected with either (left panel) m5-HT3A subunit cDNA, and probed with pAb120 antibody (1:300) or (right panel) m5-HT3A and h5-HT3B cDNA and probed with pAb77. Scale bars represent 10 μM.