Figure 5

FO inhibits viral propagation in cell culture in a dose dependent manner. (A) Infection of MDBK monolayers with PrV-Cam without FO produced large plaques with lysed cells in the central region (picture shows a border of a plaque). (B) With 70 μg/ml FO, no plaques were formed and fluorescent cells appeared in groups of 20 – 40. (C) Presence of 400 μg/ml resulted in single fluorescent cells without viral spread to neighbouring cells. (D) Traced TGN (red) in cell culture two days after inoculating PrV-614 intranasally and one day after plating. Four hundred μg/ml FO efficiently inhibited viral spread. Cells were loaded with the calcium sensitive dye Fluo-4 (green). Red and green fluorescences were merged in this figure. Arrowhead indicates an infected glial cell. Bars in A: 20 μm, D: 30 μm