Figure 5
From: Isoflurane depresses hippocampal CA1 glutamate nerve terminals without inhibiting fiber volleys
Insensitivity of somatic spike currents to isoflurane. Control traces at left are four overlaid leak-subtracted inward spikes. The records are from a representative whole-cell perforated patch in the pyramidal cell body layer of the hippocampal CA1 region. 100 ms voltage pulses to -30 mV were applied at 10 s intervals to elicit spikes (VH = -70 mV). Currents in green show four spikes recorded after a 15 min exposure to 60 nM TTX. At right are overlays of spike currents recorded before and after a 10 min exposure to 350 μM isoflurane (1 MAC). The blue traces were recorded after exposure to isoflurane.