Figure 3From: Neural stem cells from protein tyrosine phosphatase sigma knockout mice generate an altered neuronal phenotype in cultureNeurons show enhanced migration patterns and neurite outgrowth when differentiating from neurospheres derived from PTPσ (-/-) mice. All images taken after 7 days of differentiation. Epi-fluorescence with the β-III tubulin antibody. A. Morphological appearance: a) Appearance of neurospheres from PTPσ(+/+) mice grown on laminin coated coverslips. Scale bar = 150 μm. b) Higher magnification of (a) to show neuronal progeny derived from the PTPσ(+/+) genotype. Scale bar = 100 μm c) Appearance of the neuronal migration from PTPσ(+/-) derived neurospheres. Scale Bar = 100 μm d) Neurons that differentiate from neurospheres from a PTPσ(-/-) mouse appear extensively distanced from the neurosphere of origin. Scale bar = 100μm. e) Neuronal differentiation from a PTPσ(+/-) neurosphere. Scale bar = 50μm. f) Long random processes characterize PTPσ(-/-) neurons. β-III tubulin immunocytochemistry. Scale bar = 50μm. g) High power view of a long process from a neuron derived from a PTPσ(-/-) neurosphere. β-III tubulin immunocytochemistry. Scale bar = 25μm.Back to article page