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Figure 6 | BMC Neuroscience

Figure 6

From: Dopamine presynaptically and heterogeneously modulates nucleus accumbens medium-spiny neuron GABA synapses in vitro

Figure 6

DA receptor immunostaining shows a presynaptic pattern. A. D1 immunostaining. (A1) A field in a nAcc culture is shown with fluorescence superimposed on a differential interference contrast (DIC) image. Here five of six cells were D1 immunoreactive. Note that the cell body staining did not extend reliably out onto the dendrites. Rather, punctate or linear staining was seen in the neuropil, consistent with staining of axons and presynaptic varicosities. Regions of interest outlined in red and blue are shown at 2× magnification on the right side. (A2) D1 fluorescence revealed strings of varicosities studding a thin process. (A3) In another region of the neuropil, continuous staining of putative-axonal processes was seen. B. D2 immunostaining. (B1) A field in a different nAcc culture containing four neurons, two of which were D2 immunoreactive, is shown. Again, note that the cell body staining does not extend continuously out onto the dendritic processes. Rather, punctate and linear staining of putative axonal processes is evident in the neuropil. (B2) Several varicosities without clear intervening axonal staining are seen studding unstained dendrites. A stretch of labeled axon is seen on the lower, right corner of the field. (B3) In another region, there were stained varicosities studding axonal processes. Note the dearth of postsynaptic dendritic labeling.

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