Table 1 Primary antibodies and lectins
Antigen | Host | Dilution | Source (catalog #; clone #) | Reference |
|---|---|---|---|---|
Syntaxin 1 | Mouse | 1:500–1:1000 | Sigma Chemical Company, St. Louis, MO (S0664; clone HPC-1) | 57 |
Syntaxin 2 | Rabbit | 1:100–1:200 | Synaptic Systems, Göttingen, Germany (110-022) | 108 |
Syntaxin 3 | Rabbit | 1:750–1:1000 | Novus Biologicals, Littleton, CO (AB4113) | 5,109 |
Syntaxin 4 | Rabbit | 1:100–1:200 | Chemicon International, Temecula, CA (AB5330) | 95,110 |
Bassoon | Mouse | 1:500 | Stressgen Biotechnologies, Victoria, British Columbia, Canada (VAM-PS003; clone SAP7F407) | 111 |
Calbindin | Mouse | 1:300 | Sigma Chemical Company, St. Louis, MO (C9848; clone CB955) | --- |
CD15 | Mouse (IgM) | 1:50 | BD Biosciences Pharmingen, San Diego, CA (555400; cloneHI98) | 35 |
Goα | Mouse | 1:500–1:1,000 | Chemicon International, Temecula, CA (MAB3073; clone 2A) | 112 |
Glutamic Acid Decarboxylase, 65 kDa (GAD-65) | Mouse | 1:500–1:1000 | Chemicon International, Temecula, CA (MAB351; clone GAD-6) | 113 |
Glycine transporter 1 (GlyT1) | Goat | 1:2000 | Chemicon International, Temecula, CA (AB1771) | --- |
Glutamine synthetase | Mouse | 1:1000 | Chemicon International, Temecula, CA (MAB302; clone GS6) | 114 |
Kinesin II | Mouse | 1:50 | Covance Inc., Princeton, NJ (MMS198P; clone K2.4) | 115 |
Microtubule Associated Protein 1 (MAP-1) | Mouse | 1:300–1:500 | Sigma Chemical Company, St. Louis, MO (M4278; clone HM-1) | 116 |
Peanut Agglutinin (PNA) | --- | 1:10 | Molecular Probes, Eugene OR | 117 |
Postsynaptic density protein-95 Kda (PSD-95) | Mouse | 1:100 | Upstate Biotechnologies, Lake Placid, NY (05–494; clone K28/43) | --- |
Synaptic vesicle protein 2 (SV2) | Mouse | 1:20–1:100 | Dr. K Buckley, Harvard Medical School, Boston, MA | 118 |
Synapsin I | Mouse | 1:500–1:1000 | Chemicon International, Temecula, CA (MAB355) | 119 |
Tyrosine hydroxylase (TH) | Mouse | 1:500 | Chemicon International, Temecula, CA (MAB318; clone LNC1) | --- |
Vesicular glutamate transporter 1 (VGLUT1) | Guinea pig | 1:2500–1:5000 | Chemicon International, Temecula, CA (AB5905) | 28 |
Vesicular glutamate transporter 3 (VGLUT3) | Guinea pig | 1:2500 | Chemicon International, Temecula, CA (AB5421) | 26 |