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Figure 3 | BMC Neuroscience

Figure 3

From: Reduced neuronal cell death after experimental brain injury in mice lacking a functional alternative pathway of complement activation

Figure 3

Attenuated neuronal cell death in the injured hemisphere of factor B gene-deficient mice 24 hours after closed head injury. Coronal cryosections of the left (injured) hemisphere of wild-type (fB+/+, panels A-E) and factor B knockout mice (fB-/-, panels F-J) were analyzed by immunohistochemistry with a specific antibody to the neuronal marker NeuN (A, B, F, G) or by TUNEL-histochemistry of adjacent sections (D, E, I, J). The overall cellular morphology of the TUNEL sections is revealed by DAPI nuclear stain (C, H). The panels B, E, G, J represent a 4-fold magnification of the respective panels A, D, F, I. The evident destruction of the cortical tissue in brain-injured wild-type mice (upper panels) appears to be structurally preserved in fB-/- mice (bottom panels). The data shown here are highly reproducible in all tissue sections and animals assessed. Original magnifications: 100× (A, C, D, F, H, I), 400× (B, E, G, J).

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