Figure 5
From: Short G-rich oligonucleotides as a potential therapeutic for Huntington's Disease
Specificity of monotonic oligonucleotides in the inhibition of mutant Htt fragment aggregation. Various monotonic 20-mers were tested for inhibitory activity in the assay outlined in Figure 1. HDC, 20-mer with all Cs; HDA, 20-mer with all As; HDT, 20-mer with all T's; HDG, 20-mer with all Gs. Four independent experiments were carried out in duplicate and this blot is most representative of all of the results. 0 hour, reaction stopped at zero time point; 24 hour, reaction lacking oligonucleotides, stopped at 24 hours; Congo Red, incubation with 10 μM of Congo Red for 24 hours.