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Table 4 Animal utilisation

From: Effects of lipopolysaccharide-induced inflammation on expression of growth-associated genes by corticospinal neurons

Survival time

Cortical LPS (with SCI) for IHC

Cortical LPS with retrograde labelling

Cortical LPS for ISH

Cortical LPS with SCI and anterograde labelling

SCI with anterograde labelling

Controls (Gelfoam not applied)

1 day

3

     

3 days

5 (+3)

5

4 (+1 control)

  

3 (+3)

7 days

4 (+2)

 

4

  

1

14 days

2 (+2)

2

3

  

2

21 days

   

4

4

 

1 month

2 (+2)

1

4

   

TOTAL

16 (+9) = 25

8

15 (+1)

4

4

6 (+3)

  1. Notes: IHC = immunohistochemistry; ISH = in situ hybridisation; SCI = spinal cord injury (denotes animals with unilateral transection of corticospinal tract at C4).
  2. Controls were animals in which the dura was opened unilaterally without application of LPS (in 6 of these animals Gelfoam was applied to the cortex; in 3 others no Gelfoam was applied). In addition, control exposure of the left cerebral cortex was carried out in all animals with unilateral LPS application.
  3. This Table does not include 6 positive control animals in which the expression of growth associated proteins was examined in the facial nucleus following facial nerve injury and it also excludes 12 animals used in pilot studies to determine the optimal dose of LPS.