Analysis of gene expression during neurite outgrowth and regeneration

BMC Neuroscience

Table 1 Validation using quantitative real-time PCR (qPCR) of genes found to change significantly in DRG by microarray.

Gene	Gene Title	Max. fold change by microarray^§	Max. fold change by qPCR	p-value
Cntn3	Contactin 3	2.6	11.0	0.007
Col18	Procollagen, type XVIII, alpha 1	4.3	8.1	0.0003
Cryab*	Crystallin, alpha B	123.0	42.5	<.0001
Fdps	Farnesyl diphosphate synthetase	2.4	2.0	<.0001
Gli2*	GLI-Kruppel family member GLI2	1.8	2.4	0.03**
Sparc	Secreted acidic cysteine rich glycoprotein	4.8	6.5	<.0001
Timp3*	Tissue inhibitor of metalloproteinase 3	2.6	3.6	0.003
Tubb3	Tubulin, beta 3	1.6	2.6	0.0002

p-value indicates significance of qPCR data for a quadratic model of change over time.
* Selection of these genes included probesets not part of the matched DRG-SCG dataset, as an additional screen for genes missed by these filters.
** Significance for this gene is for a linear model of change over time; this gene does not fit a quadratic model.
^§ Changes observed by microarray were statistically significant (quadratic model; p ≤ 0.05).

ISSN: 1471-2202