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Figure 3 | BMC Neuroscience

Figure 3

From: AVPV neurons containing estrogen receptor-beta in adult male rats are influenced by soy isoflavones

Figure 3

Representative photomicrographs of apoptotic cells (brown nuclear staining), astrocytes (red) and neurons (green) by TUNEL and dual GFAP/NeuN immunofluorescent staining in AVPV of 150 day-old male Long-Evans rats. All the photomicrographs (coronal brain sections) are from animals fed an isoflavone-rich diet (Phyto-600) from conception until time tissue was collected. An astrocyte (red; arrow) is indicated in A, whereas the neurons are displayed in green. B is a representative photomicrograph of TUNEL staining in AVPV (n = 5). C displays dual GFAP/NeuN immunofluoresent stained sections within AVPV, which is 6 μm apart from B. The apoptotic cells (arrows in B) were identified as neurons (arrows in C). Bar = 25 μm for all the photomicrographs.

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