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Figure 5 | BMC Neuroscience

Figure 5

From: AVPV neurons containing estrogen receptor-beta in adult male rats are influenced by soy isoflavones

Figure 5

Representative photomicrographs of apoptotic cells (TUNEL) and ERalpha-immunoreactive cells (ERalpha IHC) in AVPV of 150 day-old male Long-Evans rats. All the photomicrographs (coronal brain sections) are from animals fed an isoflavone-rich diet (Phyto-600) from conception until time tissue collected. ERalpha-immunoreactive (ir) cells were visualized with diaminobenzadine (DAB) and seen as brown nuclear staining (arrows in ERalpha). Sections were counterstained with hematoxylin (blue). A, C, E, G and I are representative photomicrographs of TUNEL staining in AVPV (n = 5). B, D, F, H and J are ERalpha IHC stained sections within AVPV, which are 6 μm apart from A, C, E, G, and I, respectively. Apoptotic cells (stars in TUNEL) were not ERalpha-ir (stars in ERalpha). Bar = 25 μm for all the photomicrographs.

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