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Figure 7 | BMC Neuroscience

Figure 7

From: Intracerebroventricular antisense knockdown of Gαi2 results in ciliary stasis and ventricular dilatation in the rat

Figure 7

i2 immunostaining imaged by confocal microscopy. Upper row, reference antibody against Gαi2 (Dr. Tomiko Asano); lower row, commercial antibody against Gαi2 (red) and Calbindin D-28 (green). Both antibodies show intense staining of ependymal cilia in ODN control (nonsense oligodeoxynucleotide) and saline control groups and attenuation of Gαi2 signal in AS-ODN (antisense-oligodeoxynucleotide against Gαi2) treated animals. Background staining with Calbindin D-28k (CaBP) was used to better visualize intracellular staining in endoplasmic reticulum with Chemicon antibody. Scale bar: 10 μm.

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