Figure 2

Protection from Cisplatin induced neurite toxicity by PXD. Differentiated PC12 cells were incubated with Cisplatin alone or in combination with PXD for 24 hrs and the percentage of cells with neurites was determined. A) PXD alone had no effect on neurite outgrowth up to a concentration of 1 μM, however 10 μM PXD showed neurite toxicity (^#p < 0.001). B) Cisplatin (1 μg/ml) showed a non-significant (n.s.) trend for moderate toxicity and at 20 μg/ml showed strong neurite toxicity (***p < 0.001). PXD blocked the Cisplatin induced neurite toxicity at 100 nM (p < 0.01) and 1 μM (p < 0.001), while the combination of 10 μM PXD and Cisplatin at 1 μg/ml or 20 μg/ml showed enhanced neurite toxicity compared to 10 μM PXD alone (p < 0.01 and ^##p < 0.001 respectively, combined results of n = 3 experiments).