Figure 3From: Dynamic remodelling of synapses can occur in the absence of the parent cell bodySome nerve terminals and muscle endplates fragmented in response to BzATP. All preparations were fixed in 4% paraformaldehyde prior to immunostaining for neurofilament 165 and SV2 (green: axons and terminals), visualised with a Cy2-conjugated secondary antibody and co-stained with TRITC-αBTX (red: muscle endplates), 165 min after a 15 min BzATP (100 μM) pulse. Nerve terminals associated with either fragmented (a), or absent (b) muscle endplates were seen, as were fragmented nerve terminals contacting either apparently normal (c), or fragmented endplates (c, d). Scale bar a, d = 15 μm, b, c = 30 μmBack to article page