Figure 7
From: Amyloid-like aggregates of neuronal tau induced by formaldehyde promote apoptosis of neuronal cells
Hoechst staining of cells in the presence of amyloid-like tau. Treatment with formaldehyde-treated tau resulted in apoptotic death. Note that formaldehyde was removed from the protein samples by ultrafiltration. SH-SY5Y cells were treated for 3 days with tau deposits (2 μM) induced by pretreatment with 0.1% formaldehyde. The cells were collected and stained with Hoechst 33258. Nuclei were visualized by fluorescence microscopy. Bar: 25 μm. (A) Control culture in DMEM without serum. (B) Cells treated with self-aggregated tau. (C) Cells treated with 0.1 %-acetaldehyde-treated tau. (D) Cells treated for 72 h with the mock solution without formaldehyde were collected and stained with Hoechst 33258. (E) Cells treated with 0.1 %-formaldehyde-treated tau. The arrows designate the presence of apoptotic nuclear profiles. Data are expressed as a percentage of the control (cells treated with vehicle alone) and presented as the mean ± SEM (n = 6). (F) Statistics of apoptotic cells from A-E.