Figure 14

Panels (A and B) show examples of immuno-electron microscopy with anti Necl-3 antibody in the pons of three-month old rats. The signal was enhanced with nanogold particles. Note the immunoreactivity at the interface between the axon shaft (star) and the concentric myelin folds. The myelin sheaths locally detach from the axon due to the fixation and embedding procedures. Panel (C and D) shows representative fields of types of cells attached to the control IgGs (C) or to Fc-Necl-3 treated plates (D). Panel (E and F), Fc-Necl-3 bound to an oligodendrocyte. The phase contrast picture shows a typical oligodendrocyte as well as another cell (*), probably an astrocyte (Panel E). The image in the red-pass channel shows that, unlike the astrocyte (*), the oligodendrocyte binds to Fc-Necl-3, as revealed by a rhodamine-coupled secondary antibody (Panel F). Panel (G) The numbers of oligodendrocytes per field (Y-axis) were scored in control dishes (ctrl Ig) and Fc-Necl-3 coated dishes (error bars represent 1 standard deviation, P(T≤t) = 0.018)