- Open Access
Factors that regulate embryonic gustatory development
© Krimm; licensee BioMed Central Ltd. 2007
- Published: 18 September 2007
Numerous molecular factors orchestrate the development of the peripheral taste system. The unique anatomy/function of the taste system makes this system ideal for understanding the mechanisms by which these factors function; yet the taste system is underutilized for this role. This review focuses on some of the many factors that are known to regulate gustatory development, and discusses a few topics where more work is needed. Some attention is given to factors that regulate epibranchial placode formation, since gustatory neurons are thought to be primarily derived from this region. Epibranchial placodes appear to arise from a pan-placodal region and a number of regulatory factors control the differentiation of individual placodes. Gustatory neuron differentiation is regulated by a series of transcription factors and perhaps bone morphongenic proteins (BMP). As neurons differentiate, they also proliferate such that their numbers exceed those in the adult, and this is followed by developmental death. Some of these cell-cycling events are regulated by neurotrophins. After gustatory neurons become post-mitotic, axon outgrowth occurs. Axons are guided by multiple chemoattractive and chemorepulsive factors, including semaphorins, to the tongue epithelium. Brain derived neurotrophic factor (BDNF), functions as a targeting factor in the final stages of axon guidance and is required for gustatory axons to find and innervate taste epithelium. Numerous factors are involved in the development of gustatory papillae including Sox-2, Sonic hedge hog and Wnt-β-catenin signaling. It is likely that just as many factors regulate taste bud differentiation; however, these factors have not yet been identified. Studies examining the molecular factors that regulate terminal field formation in the nucleus of the solitary tract are also lacking. However, it is possible that some of the factors that regulate geniculate ganglion development, outgrowth, guidance and targeting of peripheral axons may have the same functions in the gustatory CNS.
- Brain Derive Neurotrophic Factor
- Geniculate Ganglion
- Fungiform Papilla
- Taste System
- Target Innervation
The unique morphology of the taste system makes it ideal for the study of the molecular factors regulating sensory development. For example, mammalian lingual taste buds develop within specialized structures called papillae, which are located in a specific spatial array. These fungiform papillae provide discrete targets for innervating neurons, making this system ideal for examining factors regulating neuronal targeting during development. It is also the case that gustatory neurons project to specific regions of the oral cavity that contain taste buds (tongue and palate); therefore, unlike nociceptors or mechanoreceptors they can be identified with retrograde tracers. In addition many of the same factors that regulate gustatory development (like BDNF) may also be important for CNS development and/or function. In spite of the multiple advantages that the taste system provides to a general understanding of developmental neurobiology, it has not been widely studied. Some important factors regulating taste system development have been identified. However, the majority of these regulatory factors and the mechanisms by which they function are largely unknown.
Timeline of morphological development in the taste system.
Placode delamination migration
Initial axon outgrowth
Peak cell production
Axons reach tongue
Peak cell death
Tongue and taste buds
Fungiform papillae (placode) (SEM)
Full no. of fungiform papillae
Taste bud differentiation begins
Full no. of vallate taste buds
Induction and formation of epibranchial placodes
Epibranchial placodes are transient ectodermal thickenings. The first epibranchial placode differentiates to form the geniculate ganglion, and the second forms the petrosal ganglion. There is accumulating evidence that all cranial placodes, including the epibranchial placodes, arise from a common placodal primordium . Early on, the Six1/2 and Six4/5 subfamilies and the Eya family of transcription factors are expressed in the horseshoe-shaped area of pre-placodal region. Disruption of any of these genes disrupts the development of multiple placodes. Therefore, these genes may regulate multiple placodal properties. The epibranchial placodes are among those affected by mutations in the Eya family. In Eya1 null mice, the development of the geniculate and petrosal ganglia is completely blocked , and both ganglia fail to express important downstream differentiation factors. The early arrest of the differentiation program in these ganglia causes the cells to undergo apoptosis. Six1 interacts with Eya1 and is not expressed in Eya1-/- mice. Six1 gene mutations, which have less severe effects than Eya1 mutations, result in the absence of the geniculate ganglion and a partial loss of the petrosal ganglion. Therefore, members of the Six1/2 and Six4/5 subfamilies and the Eya family are required for geniculate and petrosal ganglion development and may be important for conferring a pan-placodal fate in developing ectoderm.
Induction of individual placodes from the common placodal primordium is likely to be a complex multistage process . Following expression of genes in the Six and Eya families, other transcription factors are expressed in multiple placodes in partially overlapping patterns. These factors likely define subsets of placodes. One set of factors that could confer placodal identity are the Pax genes. Pax2 and Pax8 are expressed in the posterior placodal region, where the epibranchial and otic placodes are derived . Pax2 may regulate epibranchial neuron identity  and is important for otic placode development . Because Pax2 expression is more restricted than the pan-placodally expressed genes, it may be important for conferring placodal identity. However, since Pax2 expression is present in both the epibranchial and otic placodes, it must cooperate with other factors to specify epibranchial placode identity.
In addition to transcription factors mentioned above, signals arising from the pharyngeal pouch are also important for epibranchial placode formation . Included among these signals are members of the bone morphogenic protein family (BMP). The pharyngeal pouch endoderm expresses BMP7, which has been shown to induce the formation of epibranchial placodes when ectopically expressed in a chick embryo. Interestingly, removal of functional BMP7 in mice causes deficits that are restricted to the developing eye and kidney [14, 15]. Therefore, it is possible that BMP7 is not important for epibranchial placode formation in mice or more likely, that another BMP family member functions redundantly with BMP7 to induce epibranchial placode formation [15, 16]. BMP7 expression is unaffected by absence of Eya1 . Thus, BMP signalling is independent of early pan-placodal transcription factors like Eya1.
Neuronal specification within the epibranchial placodes
Cells of the epibranchial placodes differentiate into neuroblasts and subsequently delaminate (occurring on E9 in mice), migrate, and coalesce to form the geniculate and petrosal ganglia . In general, placodal neuronal differentiation is under the control of the basic-helix-loop-helix (bHLH) transcription factors, which are related to the Drosophila atonal and achaete-scute genes . One such bHLH transcription factor is neurogenin 2 (Ngn2). Ngn2 expression is dependent on Eya1  and strong Ngn2 expression is first observed in the geniculate placode at E8.5 and in the petrosal placode at E9.0 , while the related factor Ngn1 is only weakly expressed. The placodal neuroblasts that give rise to the geniculate and petrosal ganglia fail to delaminate and migrate in the absence of Ngn2 . These neuroblasts also fail to express neuron differentiation markers (e.g., neurofilaments), indicating that Ngn2 regulates neuronal fate determination for the geniculate and petrosal ganglia. Ngn1 does not appear to share this function as the development of these ganglia is unaffected by Ngn1 knockout . However, Ngn1 is required for the development of the trigeminal and otic ganglia .
In addition to Ngn2, the homeodomain transcription factors, Phox2a and Phox2b, regulate pan-neuronal fate in the geniculate and petrosal ganglia. Phox2a expression precedes Phox2b expression in the geniculate and petrosal ganglia. Accordingly, Phox2a expression is dependent on Eya1, but is independent of Ngn2, and Phox2b expression is dependent on Ngn2 [11, 19]. Studies of Phox2a null animals reveal that the geniculate and the petrosal ganglia atrophy in the absence of this transcription factor . Moreover, in the absence of Phox2b, the geniculate and petrosal ganglia degenerate . Thus, both Phox2 genes are clearly necessary for the continued differentiation of the geniculate and petrosal ganglia.
Differentiation of gustatory specific cell traits
The factors discussed so far confer a neuronal fate to epibranchial placode derived neurons, most of which are visceral sensory neurons. Since development of the trigeminal ganglion is regulated by a different set of factors, it could be argued that these factors specifically regulate visceral sensory fate rather than pan-neuronal fate. However, the visceral sensory neurons of the geniculate and petrosal ganglia are made up of several neuronal subpopulations. For example, in addition to gustatory neurons, the petrosal ganglion also contains baroreceptive neurons. Moreover, the gustatory neuron population can be divided further based on the taste bud population they innervate and their physiological response characteristics. Currently, nothing is known about how the differentiation of specific neuron sub-phenotypes in the geniculate and petrosal ganglia is regulated. However, the following possibilities are likely.
Coordinated expression of an, as yet, unidentified family of transcription factors may confer specialized gustatory neuron phenotypes. There is precedence for this scenario in other sensory ganglia like the dorsal root ganglia (DRG). In the DRG, the transcription factor Runx1 regulates the development of channels and receptors that transduce pain. A related factor, Runx3, regulates proprioceptive neuron differentiation [22, 23]. A similar mechanism may underlie regulation of visceral neuron subtype differentiation in the geniculate and petrosal ganglia. Large-scale expression mapping  has been used to identify transcription factors regulating somatosensory neuron subtypes. A similar approach could prove useful in the identification of transcription factors expressed in and capable of regulating the differentiation of geniculate and petrosal subpopulations.
The differentiation of specific neuron sub-phenotypes may also be regulated by the same factors that act early in the general differentiation of neurons. It is possible that the expression of these factors becomes restricted to specific subpopulations of neurons within the geniculate and petrosal ganglia as development proceeds. These factors may then regulate differentiation of neuronal subpopulations. There is evidence to support this model of neuron subtype differentiation. For example, Phox2 genes, which play a role in early neuronal differentiation, have been shown to impart cell-specific traits to geniculate and petrosal neurons. During embryonic development, both the geniculate and petrosal ganglia temporarily adopt a noradrenergic phenotype, which requires Phox2a expression . Phox2a is also required for the development of another cellular trait, the expression of a receptor subunit (c-Ret) for the glial-derived family of neurotrophins. Later in embryonic development (E16.5), co expression of Phox2a and Phox2b defines a population of neurons in the petrosal ganglion that expresses the dopamine-synthesizing enzyme, tyrosine hydroxylase (TH), in response to depolarizing stimuli . These neurons are the chemoafferents that innervate the carotid body and are important for regulating breathing. Unfortunately, none of the neuronal traits examined thus far are relevant to a taste neuron phenotype, and there is no evidence to support a role for Phox2 genes in the specific regulation of taste neuron differentiation.
Gustatory ganglia cell cycle dynamics
Following migration, a transit-amplifying population of neuronally committed cells (i.e., neuronal precursors) continue to proliferate, resulting in ganglion expansion . A balance between the number of cells that are initially born in the ganglia, the number that differentiate into neurons, and the number that die determines the final number of neurons within a ganglion. For the geniculate ganglion, neuron production peaks at approximately E12 in rats , which is roughly equivalent to E10 in mice. However, proliferation occurs over a fairly prolonged period. It is not known how or if terminal mitosis relates to neuronal phenotype. For example, are gustatory neurons of the geniculate generated before, after, or at the same time as somatosensory neurons innervating the external ear? Are palatal and tongue gustatory neurons generated at different times? These questions can be addressed by techniques that allow the precise temporal discrimination of when terminal mitosis occurs . Knowing if subpopulations are generated at the same or different times may provide incites into the factors that regulate their specification as well as those that control cell proliferation for these neurons. It is also not known how many stem cells contribute the formation of the gustatory ganglia or whether or not clonally related precursors contribute to multiple or only one neuronal subpopulation.
Typically, during development, neurons are overproduced and ganglia undergo a period of developmental cell death. In the geniculate ganglion, the total number of neurons remains fairly constant across embryonic age, indicating that new neurons are differentiating at the same rate as others are dying . Neuronal death reaches its peak at E16.5 in rat, which approximates E14.5 in mice. It is at this embryonic age that gustatory fibers first penetrate the epithelial surface of fungiform papillae to form neural buds [32–34]. The finding that geniculate neuron death peaks during target innervation is consistent with the possibility that factors produced by fungiform papillae regulate neuron survival.
One set of factors, produced by neuronal targets and known to regulate sensory neuron survival, are the neurotrophins . The neurotrophins are a group of structurally and functionally related growth factors. There are four members of the neurotrophin family in mammals: nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT3), and neurotrophin-4 (NT4/5). In addition to their classic target-derived role, neurotrophins are produced in and near sensory ganglion where they influence neuronal cell cycle kinetics and differentiation [27, 36–41].
Taste buds require innervation for their maintenance [46–48]. Consequently, fungiform papillae and taste buds are lost in Bdnf-/-and Ntf5-/- mice, showing that both BDNF and NT4/5 support gustatory neurons of the geniculate [49–52]. On the other hand, circumvallate taste buds are lost in only in Bdnf-/-mice [49, 51, 53]. Thus, BDNF, not NT4/5, is required for petrosal gustatory neuron survival. No fungiform papillae are lost in Ntf3-/- mice . However, Bdnf-/-/Ntf3-/- animals exhibit more taste bud loss than Bdnf-/- animals , leaving it unclear whether NT3 regulates gustatory neuron number and/or taste buds. It is also unclear whether taste buds are lost solely because of a loss of neurons in neurotrophin mutants. That is, neurotrophins produced in the tongue may have autocrine or paracrine effects on taste bud development or maintenance.
The source of the neurotrophins influencing gustatory ganglion development is presently unknown. BDNF and NT3 are expressed in gustatory papillae, taste buds, geniculate ganglia, and in the rostral nucleus of the solitary tract (NTS) [51, 55–61]. Although less well studied, NT4/5 expression has been observed in the tongue, taste buds, and in neurons in the geniculate ganglion [62–64]. The presence of neurotrophins in these various locations within the taste system, suggests that they would be capable of regulating cell cycle events at a wide range of embryonic time points.
Very little data is available regarding the mechanisms by which BDNF and NT4/5 regulate the proliferation and death of neurons in the petrosal and geniculate ganglia. Neuron loss in the petrosal ganglion of Bdnf-/- mice has been shown to require the proapoptotic gene Bax, indicating that these neurons undergo apoptosis in the absence of BDNF . BDNF probably functions to block cell death by a similar mechanism in geniculate neurons, although the geniculate has not yet been examined in double Bdnf-/-/Bax-/-mice. It is also not yet clear which cells types are dying in BDNF (i.e. differentiated neurons or neuronal precursors). The specific role(s) of NT4/5 is even less clear. The earlier loss of geniculate neurons in Ntf5-/- mice compared with Bdnf-/- mice, could be due to NT4/5 regulation of proliferation or exit from the cell cycle. Also, a very different population of cells may be regulated early in development than is affected later in development by removal of NT4/5. Neither the timing nor the specific role of NT3 for geniculate neurons, have been examined.
It is worth mentioning that growth factors other than the neurotrophins may regulate geniculate neuron cell cycle events. For example, geniculate neurons express receptors for members of the GDNF family . Although it is not clear whether these factors influence geniculate neuron development, removal of GDNF does reduce the number of neurons in the nodose/petrosal ganglion . It remains unclear, however, whether any of these neurons innervate taste buds. Finally, one cannot ignore the possibility that all of the growth factors discussed so far may regulate neuron survival but not proliferation. If this is the case, then it is not known what factors may mediate developmental changes in proliferation in the gustatory ganglia.
Gustatory axon outgrowth and guidance
Neurite extension occurs as neuronal precursors become post-mitotic and differentiate. In vitro, geniculate axon outgrowth requires the addition of a neurotrophin to the culture media. BDNF, NT4/5, and GDNF are capable of supporting neurite outgrowth, while NT3 and NGF are not . Interestingly, the removal of BDNF or NT4/5 does not disrupt the ability of chorda tympani axons to reach the tongue . These findings indicate that while a neurotrophin is required for geniculate axon outgrowth, neurotrophins are capable functioning redundantly in vivo to support the growth of axons.
Axons of the chorda tympani must navigate the distance from the geniculate ganglion to the lingual epithelium of the dorsal tongue. These axons grow into the tongue as it develops (E12) [33, 72] and approach the epithelial surface by E13.5. Because chorda tympani axons follow precise, spatially restricted pathways to the tongue surface, a series of molecular cues from the environment must guide these axons to the lingual epithelium . It is likely that multiple attractive and repulsive cues are required to ensure that gustatory axons maintain the proper path from the ganglion to the lingual epithelium .
Multiple families of well-established axon guidance cues  exist and include the netrins, slits, semaphorins, and ephrins. While any of these factors may regulate axon guidance in gustatory neurons, most remain un-investigated in the taste system. One exception is the chemorepulsive factor, semaphorin 3A (Sema3A). Sema3A is expressed in developing tongue  and appears to be important during both trigeminal and chorda tympani axon guidance [70, 72]. Sema3A expression decreases from the medial to lateral tongue surface and prevents premature and aberrant growth of trigeminal and gustatory fibers into the tongue mid-region. In addition, as geniculate axons near the epithelial surface, Sema3A prevents premature penetration of the epithelium [77, 78]. Another member of this family, Sema3F, is also expressed by lingual epithelium, although its function remains unclear .
While semaphorins may be the primary chemorepellent molecules used by chorda tympani axons, multiple chemoattractants are undoubtedly also required to guide chorda tympani axons to fungiform papillae. For example, factors produced by the tongue may encourage initial tongue innervation. Other factors produced by the lingual epithelium could attract chorda tympani fibers to the dorsal epithelial surface. Consistent with the idea that the tongue contains chemoattractants, the axolotl oropharyngeal endoderm, which gives rise to taste buds, is chemoattractive for early gustatory neurons . Flank ectoderm is also initially chemoattractive. While pharyngeal ectoderm retains its ability to attract gustatory neurons during taste system development, the flank ectoderm loses its attractive qualities. Perhaps gene expression profiling of these two tissues across different developmental time points will enable the identification of important chemoattractant factors. Not surprisingly, the mammalian tongue is also chemoattractive ; however, virtually nothing is known about the factor responsible for its chemoattractive properties or if it has any similarity to the amphibian tongue chemoattractant. In vitro experiments reveal that the chemoattractive properties of the mammalian tongue are not affected by the presence of BDNF or NT4/5, ruling out the possibility that the chemoattractant is one of these neurotrophins . Obviously, isolating the tongue chemoattractant factor(s) is an important next step in determining how gustatory axons navigate from the gustatory ganglia into the base of the tongue and toward the lingual epithelium. Future experiments should also address whether the soft palate is as chemoattractive as the tongue for geniculate neurons and whether the tongue is chemoattractive for petrosal neurons.
The identification and functional evaluation of the guidance cues important for the taste system may be complicated by the possibility that these cues may interact with other environmental factors, like neurotrophins, to produce a unique effect. For instance, NT4/5, but not BDNF, enhances the responses of geniculate neurons to Sema3A and Sema3F . In mice that over express NT4/5 in the epithelium, chorda tympani fibers remain below the surface as if repelled by the lingual epithelium . Because Sema3A normally inhibits chorda tympani innervation of the lingual epithelium , NT4/5 repulsion could be explained, at least in part, by NT4/5-mediated enhancement of geniculate fiber responses to Sema3A. NGF, on the other hand, has been shown to reduce the sensitivity of somatosensory neurons to Sema3A [77, 81]. BDNF may act similarly by reducing the sensitivity of geniculate neurons to Sema3A inhibition, although it has not been shown to inhibit responses to Sema3A in culture .
Summary of geniculate and petrosal ganglion development
The geniculate and petrosal ganglia arise primarily from epibranchial placodes, which arise from a common placodal region. Epibranchial placode formation appears to be regulated by a series of transcription factors and signals from the pharyngeal pouch endoderm, like BMP7 (Figure 2). Six1 and Eya1 regulate specification of the pan-placodal region, and Pax2 may confer additional specification upon this region to produce the epibranchial and otic placodes. Ngn2, Phox2a, and Phox2b are all important regulators of neuronal differentiation in the geniculate and petrosal ganglia. The identity of the factors that regulate the gustatory neuron phenotype and subtypes is unknown. Likewise, it is unclear what factors may regulate neuroblast proliferation in either the geniculate or the petrosal ganglion. However, the neurotrophins BDNF, NT4/5, and perhaps NT3 are important for the survival of differentiated gustatory neurons and possibly of neuronal precursors. Neurotrophins are also required for axon outgrowth from the geniculate ganglion; however, different neurotrophic factors function redundantly such that no single neurotrophin is required. In general, very little is known about the factors important for gustatory axon guidance. While semaphorins function as important chemorepellents during gustatory axon guidance, no chemoattractants have been isolated. In addition, it is not known whether semaphorins regulate axon guidance for petrosal gustatory neurons innervating the circumvallate papillae.
Taste papilla formation
Taste buds on the tongue are located in three specialized types of epithelial structures called papillae. In mice, approximately 90 fungiform papillae (180 in rats) occupy the rostral two-thirds of the tongue, and each typically contains one taste bud. A single circumvallate papilla is located on the midline of the caudal tongue, and folds (foliate papillae) are located at the lateral edges of the caudal tongue. When the tongue initially forms, around E12 in mouse, the surface is homogenous. Placodal thickenings then arise on the surface by E13. At E13, fungiform placodal thickenings (or placodes) occupy two bilateral rows adjacent to the mid-line; however, by E14.5 a full complement of developing fungiform papillae is present on the tongue . The single circumvallate papilla, which will house taste buds on the back of the tongue, also arises at E13 as a swelling on the midline of the back of the tongue . As the development of fungiform papillae proceeds, the placodal edges extend into the underlying mesenchyme and evaginate into a raised structure. Once the basic papillae shape is established, papillae continue to differentiate. The epithelial cells (keratinocytes) at the papillary surface, for example, become squamous. On the soft palate, taste buds are not located in papillae; however, they are located in slightly raised areas (eminences) on the palatal surface. The majority of taste buds are in two long eminences, which overlie the regions of the tongue just lateral to the circumvallate papillae; these regions are the geschmacksstreifen (taste stripe) .
Signaling factors involved in epithelial patterning in numerous different tissues are also expressed on the tongue surface during development. These factors include sonic hedge hog (Shh), the bone morphogenic proteins (Bmp 2, 4), Noggin , fibroblast growth factor 8 (FGF 8) [85–87], Sox-2 , and Wnt ligands . Recently, there has been considerable progress in the understanding of how some of these factors regulate papilla morphogenesis. Because this work has been recently reviewed , I will only touch on it briefly here.
Wnts, a large ligand family, function via multiple receptor mediated pathways, one of which involves β-catenin activation which results in transcriptional activation of Lef1 and Tcf transcription factors. Activation of this pathway by addition of LiCl to tongue cultures increases placode number [90, 91]. Disruption of β-catenin signaling, by either genetic deletion of epithelial β-catenin, Lef1, Wnt10b, or overexpression of a β-catenin antagonist (dickkopf1), blocks fungiform placode development [90, 91]. A dominate stabilizing mutation of β-catenin causes a dramatic increase in papillae number, such that the tongue is completely covered by fungiform papillae . Wnt-β-catenin signalling regulates Sox-2 expression . When Sox2 expression is reduce to 20% of normal levels fungiform placodes form, but papillae fail to develop to maturity. Overexpression of Sox2 increases fungiform papillae at the expense of filiform papillae development.
In addition to regulating Sox-2, Wnt-β-catenin signaling interacts with Shh signaling , although the nature of this interaction is unclear. Although Shh knockout mice lack tongues, Shh can be functionally manipulated in tongue organ cultures using the steroidal plant alkaloids cyclopamine or jervine, or a function-blocking anti-Shh antibody [92–94]. Disruption of Shh signaling, following initial tongue development, increases the number and size of fungiform papillae on the tongue surface [92–94]. The normal pattern of fungiform papillae is also disrupted, and many fungiform papillae become fused. Pre-incubation of tongue cultures with epidermal growth factor (EGF) blocks the effects of cyclopamine on papillary number . EGF is expressed in inter-papillary regions , and when added to tongue cultures, it inhibits papilla morphogenesis in a dose-dependent manner . Thus, it may be that EGF and Shh interact with one another to regulate papillary number.
Lastly, fungiform placode development can also be inhibited by multiple BMPs. When these proteins are delivered by attaching the protein to a bead that is inserted just beneath the lingual surface, or directly in the culture medium of the E14 rat tongue, fungiform placodes fail to form the region of the bead . In contrast, the BMP antagonist, noggin, can increase the number of placodes of the tongue surface . Therefore, these factors may function together to regulate the distribution of fungiform placodes on the tongue surface. Taken together these results indicate that a large number of patterning factors function together to orchestrate the developmental pattern of fungiform papillae on the tongue surface. Much less clear are the mechanisms by which these factors function and whether or not they function upstream or downstream of each other. It is likely that future studies will focus on clarifying these issues.
Targeting of gustatory axons to fungiform papillae
To determine if BDNF is required for normal target innervation, we recently examined target innervation in mice lacking BDNF . At E14.5 and E16.5, these animals had chorda tympani fibers that branched extensively below the epithelium, but did not penetrate the epithelium and form a neural bud (Figure 6C,D). This increased branching occurred despite the fact that these mice were losing geniculate neurons between E14.5 and E16.5. Although target innervation is disrupted for some other BDNF-dependent sensory systems in Bdnf-/- mice [67, 101], this is the first demonstration of increased branching in response to BDNF removal. It is as if in the absence of BDNF gustatory fibers are hyperinnervating the tongue in order to find their targets. Eventually, four days after neural buds normally form (E18.5); a few fungiform papillae are innervated in Bdnf-/- mice. We also examined the distribution of innervation to the soft palate, where targeting is not preceded by papilla formation. Bdnf-/- mice displayed increased branching of nerve fibers in the soft palate and a loss of specific innervation to the taste bud containing areas. Therefore, BDNF is required for geniculate neurons to innervate gustatory epithelia successfully, even in the soft palate where gustatory papillae are not present. A few neural buds did finally form at E17.5, three days after they appear in wild type mice. These targeting effects are specific to Bdnf-/-mice and do not occur in Ntf5-/- mice, even through NT4/5 binds to the same receptors as BDNF. Taken together, these findings demonstrate that BDNF functions as a short range chemoattractant which allows facial gustatory neurons to locate and innervate taste epithelia during development.
Taste bud development
In amphibians, epithelial cell-cell interactions induce taste bud formation , indicating the epithelia from which taste buds differentiate contain at least one signaling factor for taste bud induction. It is also possible that epithelial cell-cell interactions are important for the early induction of taste buds in mammals, long before taste buds differentiate [1, 3]. Following the initial induction, taste bud differentiation may occur in response to a different signal, perhaps from nerve fibers . At this point, there is no experimental evidence either supporting or refuting this possibility. Experimental evidence is lacking primarily because there are no markers for mammalian taste progenitors. Without such markers, it is impossible to determine when the taste bud is first induced. Fate mapping experiments that clearly identify the population of cells that is destined to become a taste bud will be required to address this issue. Currently, taste buds can be observed only once they differentiate. We do know that, in mammals, lingual taste buds can form only within papillae, which indicates that taste bud-competent cells exist before taste buds differentiate and are restricted to gustatory papillae. It is possible that these cells become specified as gustatory placodes initially develop.
Following the initial induction and early differentiation of taste buds, some synapses between taste cells and nerve fibers can be seen . The very last feature of the taste bud to develop is the taste pore, a small hole in the keratinized epithelia of the papilla that allows solutions to access the sensory cells. Taste pores appear slowly during postnatal development . Adult taste buds are complex sensory organs with multiple cell types including supporting cells, cells capable of sensory transduction, and cells with synaptic connections to neurons [111, 112]. It is reasonable that multiple factors would be involved in the differentiation of a receptor end organ with such complexity. While the factors controlling taste system development are still unknown, multiple candidates have been identified based on their roles in other animals/systems and their expression patterns in taste buds. These factors include Shh and Nkx2.2, a factor that functions downstream of Shh and is present in some taste cells [85, 93, 113]. Transcription factors related to the Drosophila aschaete-schute complex may regulate taste bud development, as in gustatory ganglion development [113–118]. Growth factors functioning via tyrosine kinase receptors may regulate taste bud development and/or trophic maintenance of taste buds . Unfortunately, the specific roles of each of these factors in regulating taste bud development cannot be determined until multiple approaches are developed that allow for selective gene removal from developing and adult taste cells.
Summary of peripheral gustatory development
Lingual taste buds develop within specialized papillae. Multiple signaling factors are expressed by the developing placodes that will become papillae. These factors regulate the formation, size and pattern of gustatory placodes. Gustatory epithelium in the soft palate is not defined by specialized papillae. Future experiments should address whether these same factors have a similar expression pattern and role for taste bud-containing regions on the soft palate. After fungiform papillae are formed, they become innervated. BDNF expressed within fungiform papillae regulates target innervation and appears to be the primary factor that allows gustatory axons to distinguish taste from non-taste epithelia. BDNF also appears to be important for the targeting of geniculate neurons to the soft palate. However, it is not known whether gustatory neurons of the petrosal ganglion require BDNF to correctly locate circumvallate or foliate papillae. It is also not known whether BDNF is regulated downstream of factors that control fungiform placode development. The mechanisms by which taste buds are induced in mammals and the factors regulating their differentiation are still fundamentally unclear. Several transcription factors are expressed in taste buds of the circumvallate papilla and could be important for taste bud development. Further characterization of the localization of each of these factors in taste bud-containing regions could provide clues as to their specific roles in taste bud development.
In addition to innervating taste buds during development, primary gustatory neurons project to specific locations in the CNS. Central branches of gustatory axons from the geniculate and petrosal ganglia terminate in the rostral portion of the nucleus of the solitary tract (NST). The terminal field for the petrosal neurons is caudal to that of the geniculate neurons, although some overlap exists between these fields. The NST serves as an integration center for visceral sensory input to the brainstem. In rat, axons of the geniculate ganglion begin to invade the NST at E15 . By E17, geniculate terminal fields ramify in the rostral NST, and the general morphology is adult-like by E19. However, the terminal field of the chorda tympani nerve continues to increase in size through postnatal day 15 (PN15), and extensive remodeling occurs up to PN25 [121, 122]. The first synaptic thickenings in the rostral NST are detectable at E17, and the first synaptic vesicles are observed at E19 .
Outgrowth, guidance, and terminal field formation of CNS projections
A careful examination of the factors regulating the outgrowth and guidance of central gustatory axons is lacking. It is reasonable to speculate that some of the same factors that influence peripheral axon outgrowth and guidance may also be important centrally. Sema3A, for example, may function as an inhibitory molecular cue for central axon guidance, much as it does in the periphery. It is also possible that neurotrophins are important for CNS process outgrowth from the geniculate ganglion.
The ability of BDNF to regulate peripheral targeting may point to a role for this neurotrophin in the development of central targeting and terminal field innervation. Clues as to how central targeting is regulated by neurotrophins in the taste system may be gleaned from studies examining the role of neurotrophins in CNS targeting in other sensory neurons. For instance, the elimination of the pro-apoptotic BCL-2 homolog, Bax, allows sensory neurons survive in the absence of neurotrophins [123–125]. Although NT3-dependent proprioreceptive neurons survive under these conditions, they do not project far enough into the spinal cord to reach the correct location for the formation of a terminal field . This deficit is attributable to the failure of these neurons to express the transcription factor ER81, which is required for central axons to reach their targets. These data reveal that NT3 mediates the formation of proprioceptive afferent-motor neuron connections via regulation of ER81. Interestingly, not all sensory neurons require neurotrophins for CNS targeting. Sensory neurons (nociceptors) that require NGF/trkA signaling for establishing and maintaining cutaneous innervation during development do not require NGF for central targeting . Thus, it is yet to be determined whether BDNF-dependent gustatory neurons reach the NST in the absence of functional BDNF.
While very little is known about the effects of growth factors or transcription factors on terminal field development, it is clear that environmental manipulations during embryonic development influence the eventual size of the terminal field. As an example, restriction of dietary sodium during a brief embryonic period from E3 to E12 results in an enlarged gustatory terminal field by adulthood [128, 129]. This critical period for dietary effects on terminal field development occurs before chorda tympani axons reach the taste bud or the NST. This effect is not specific for sodium deprivation; protein deprivation during development can also result in enlarged gustatory terminal fields (Dr David L. Hill, personal communication). Therefore, this effect could be the result of general nutritional deficits. One possible explanation for these findings is that early dietary deprivation disrupts or enhances the developmental expression of some factor(s), like growth factors, which in turn regulate gustatory terminal field formation later in development.
Development of post-synaptic neurons in the NST
A number of studies have examined the morphological changes in postsynaptic gustatory neurons in the NST during development, but far fewer studies have examined the factors that regulate the development of these neurons. These studies reveal that some of the same factors that regulate primary sensory neuron differentiation also regulate neuronal differentiation in the rostral NST. An example is Phox2b. This transcription factor is expressed by NST neurons and neuronal precursors [21, 130] and the NST does not form in its absence . Since the gustatory ganglia degenerate in Phox2b-/- mice, Phox2b may function as a "circuit-specific" transcription factor that helps coordinate visceral sensory circuit formation.
In addition to Phox2b, neurons of the NST also express another homeobox gene, Tlx3 . In the absence of Tlx3, the Phox2b-positive neurons that will eventually form the NTS are born but are lost by E12.5. It is interesting to note that, later in development, Tlx3 expression is lost from many brainstem areas, but high expression is maintained in the rostral (gustatory) NTS, suggesting that Tlx3 may play a role in the later differentiation of neurons. In dorsal horn of the spinal cord, Tlx3 selects a glutamatergic over a GABAergic cell fate for the postsynaptic neurons receiving sensory information [132, 133]. It is possible that Tlx3 also controls the neurotransmitter fate of gustatory postsynaptic neurons in the rostral NST. Taken together, these data indicate the same set of transcriptional regulators may be required for the formation and continued differentiation of multiple components of the gustatory neuronal network. Unfortunately, it is unclear whether these transcription factors are as important for gustatory development as they are for the development of other chemoreceptors [21, 25, 134–136].
Summary of gustatory NST development
Almost nothing is known about the factors regulating central projections of gustatory axons. However, it is possible that some of the same factors that regulate peripheral axon development also influence CNS terminal field development. For example, BDNF could influence the ability of CNS gustatory neurons to terminate correctly in the NST, much as it is influences targeting in the periphery. Likewise, the transcription factors that regulate development of the geniculate and petrosal ganglia may also regulate the development of postsynaptic neurons in the NST. For example, the transcription factor Phox2b, which regulates gustatory ganglion differentiation, is required for the differentiation of NST neurons. The transcription factor Tlx3 is also expressed by rostral NST, and it may regulate the development of specific NST neuron phenotypes.
The formation of gustatory system requires a complex set of processes that are regulated by a number of different molecular cues. In recent years we have learned a fair amount about the molecular cues regulating early gustatory differentiation, neuron cell survival, gustatory papilla development and peripheral targeting. However, the specific cellular mechanisms used by these factors to regulate gustatory development are still unknown. Almost nothing is known about the factors regulating the development of gustatory neuroblast proliferation, specific gustatory neuron phenotypes, gustatory axon guidance, taste bud induction/differentiation and CNS targeting. Determining what these factors are and the mechanisms by which they function is important not only for a better understand of gustatory development, but developmental neurobiology in general.
This article has been published as part of BMC Neuroscience Volume 8 Supplement 3, 2007: The chemical senses: recent advances and new promises. The full contents of the supplement are available online at http://0-www.biomedcentral.com.brum.beds.ac.uk/1471-2202/8?issue=S3.
- Barlow LA: Gustatory System Development. The Neurobiology of Taste and Smell. Edited by: Finger TE, Silver WL, Restrepo D. 2000, New York: Wiley-Liss, 395-422.Google Scholar
- Northcutt RG, Barlow LA, Braun CB, Catania KC: Distribution and innervation of taste buds in the axolotl. Brain, behavior and evolution. 2000, 56: 123-145. 10.1159/000047200.PubMedGoogle Scholar
- Barlow LA: Toward a unified model of vertebrate taste bud development. J Comp Neurol. 2003, 457: 107-110. 10.1002/cne.10549.PubMedGoogle Scholar
- Hill DL, Mistretta CM: Developmental neurobiology of salt taste sensation. Trends Neurosci. 1990, 13: 188-195. 10.1016/0166-2236(90)90046-D.PubMedGoogle Scholar
- Mistretta CM, Hill DL: Development of the taste system: basic neurobiology. Handbook of Olfaction and Gustation. Edited by: RL D. 1995, New York: Marcel Dekker, 635-668.Google Scholar
- Mistretta CM, Hill DL: Development of the taste system: basic neurobiology. Handbook of Olfaction and Gustation. Edited by: RL D. 2003, New York: Marcel Dekker, 759-782.Google Scholar
- Northcutt RG: Taste buds: development and evolution. Brain, behavior and evolution. 2004, 64: 198-206. 10.1159/000079747.PubMedGoogle Scholar
- Oakley B, Witt M: Building sensory receptors on the tongue. J Neurocytol. 2004, 33: 631-646. 10.1007/s11068-005-3332-0.PubMedGoogle Scholar
- Barlow LA: Cranial nerve development: placodal neurons ride the crest. Curr Biol. 2002, 12: R171-173. 10.1016/S0960-9822(02)00734-0.PubMedGoogle Scholar
- Schlosser G: Induction and specification of cranial placodes. Dev Biol. 2006, 294: 303-351. 10.1016/j.ydbio.2006.03.009.PubMedGoogle Scholar
- Zou D, Silvius D, Fritzsch B, Xu PX: Eya1 and Six1 are essential for early steps of sensory neurogenesis in mammalian cranial placodes. Development. 2004, 131: 5561-5572. 10.1242/dev.01437.PubMedGoogle Scholar
- Baker CV, Bronner-Fraser M: Establishing neuronal identity in vertebrate neurogenic placodes. Development. 2000, 127: 3045-3056.PubMedGoogle Scholar
- Begbie J, Brunet JF, Rubenstein JL, Graham A: Induction of the epibranchial placodes. Development. 1999, 126: 895-902.PubMedGoogle Scholar
- Luo G, Hofmann C, Bronckers AL, Sohocki M, Bradley A, Karsenty G: BMP-7 is an inducer of nephrogenesis, and is also required for eye development and skeletal patterning. Genes Dev. 1995, 9: 2808-2820. 10.1101/gad.9.22.2808.PubMedGoogle Scholar
- Dudley AT, Lyons KM, Robertson EJ: A requirement for bone morphogenetic protein-7 during development of the mammalian kidney and eye. Genes Dev. 1995, 9: 2795-2807. 10.1101/gad.9.22.2795.PubMedGoogle Scholar
- Solloway MJ, Robertson EJ: Early embryonic lethality in Bmp5;Bmp7 double mutant mice suggests functional redundancy within the 60A subgroup. Development. 1999, 126: 1753-1768.PubMedGoogle Scholar
- Baker CV, Bronner-Fraser M: Vertebrate cranial placodes I. Embryonic induction. Dev Biol. 2001, 232: 1-61. 10.1006/dbio.2001.0156.PubMedGoogle Scholar
- Ma Q, Chen Z, del Barco Barrantes I, de la Pompa JL, Anderson DJ: Neurogenin1 is essential for the determination of neuronal precursors for proximal cranial sensory ganglia. Neuron. 1998, 20: 469-482. 10.1016/S0896-6273(00)80988-5.PubMedGoogle Scholar
- Fode C, Gradwohl G, Morin X, Dierich A, LeMeur M, Goridis C, Guillemot F: The bHLH protein NEUROGENIN 2 is a determination factor for epibranchial placode-derived sensory neurons. Neuron. 1998, 20: 483-494. 10.1016/S0896-6273(00)80989-7.PubMedGoogle Scholar
- Morin X, Cremer H, Hirsch MR, Kapur RP, Goridis C, Brunet JF: Defects in sensory and autonomic ganglia and absence of locus coeruleus in mice deficient for the homeobox gene Phox2a. Neuron. 1997, 18: 411-423. 10.1016/S0896-6273(00)81242-8.PubMedGoogle Scholar
- Dauger S, Pattyn A, Lofaso F, Gaultier C, Goridis C, Gallego J, Brunet JF: Phox2b controls the development of peripheral chemoreceptors and afferent visceral pathways. Development. 2003, 130: 6635-6642. 10.1242/dev.00866.PubMedGoogle Scholar
- Chen CL, Broom DC, Liu Y, de Nooij JC, Li Z, Cen C, Samad OA, Jessell TM, Woolf CJ, Ma Q: Runx1 determines nociceptive sensory neuron phenotype and is required for thermal and neuropathic pain. Neuron. 2006, 49: 365-377. 10.1016/j.neuron.2005.10.036.PubMedGoogle Scholar
- Kramer I, Sigrist M, de Nooij JC, Taniuchi I, Jessell TM, Arber S: A role for Runx transcription factor signaling in dorsal root ganglion sensory neuron diversification. Neuron. 2006, 49: 379-393. 10.1016/j.neuron.2006.01.008.PubMedGoogle Scholar
- Gray PA, Fu H, Luo P, Zhao Q, Yu J, Ferrari A, Tenzen T, Yuk DI, Tsung EF, Cai Z, et al: Mouse brain organization revealed through direct genome-scale TF expression analysis. Science. 2004, 306: 2255-2257. 10.1126/science.1104935.PubMedGoogle Scholar
- Brosenitsch TA, Katz DM: Expression of Phox2 transcription factors and induction of the dopaminergic phenotype in primary sensory neurons. Mol Cell Neurosci. 2002, 20: 447-457. 10.1006/mcne.2002.1135.PubMedGoogle Scholar
- Huang EJ, Reichardt LF: Neurotrophins: roles in neuronal development and function. Annu Rev Neurosci. 2001, 24: 677-736. 10.1146/annurev.neuro.24.1.677.PubMed CentralPubMedGoogle Scholar
- Liebl DJ, Klesse LJ, Tessarollo L, Wohlman T, Parada LF: Loss of brain-derived neurotrophic factor-dependent neural crest-derived sensory neurons in neurotrophin-4 mutant mice. Proc Natl Acad Sci USA. 2000, 97: 2297-2302. 10.1073/pnas.040562597.PubMed CentralPubMedGoogle Scholar
- Al-Hadlaq SM, Bradley RM, MacCallum DK, Mistretta CM: Embryonic geniculate ganglion neurons in culture have neurotrophin-specific electrophysiological properties. Neuroscience. 2003, 118: 145-159. 10.1016/S0306-4522(02)00814-X.PubMedGoogle Scholar
- Altman J, Bayer SA: Development of the cranial nerve ganglia and related nuclei in the rat. Adv Anat Embryol Cell Biol. 1982, 74: 1-90.PubMedGoogle Scholar
- Vega CJ, Peterson DA: Stem cell proliferative history in tissue revealed by temporal halogenated thymidine analog discrimination. Nature methods. 2005, 2: 167-169. 10.1038/nmeth741.PubMedGoogle Scholar
- Carr VM, Sollars SI, Farbman AI: Neuronal cell death and population dynamics in the developing rat geniculate ganglion. Neuroscience. 2005, 134: 1301-1308. 10.1016/j.neuroscience.2005.05.034.PubMedGoogle Scholar
- Lopez GF, Krimm RF: Refinement of innervation accuracy following initial targeting of peripheral gustatory fibers. J Neurobiol. 2006, 66: 1033-1043. 10.1002/neu.20289.PubMed CentralPubMedGoogle Scholar
- Mbiene JP: Taste placodes are primary targets of geniculate but not trigeminal sensory axons in mouse developing tongue. J Neurocytol. 2004, 33: 617-629. 10.1007/s11068-005-3331-1.PubMedGoogle Scholar
- Farbman AI, Mbiene JP: Early development and innervation of taste bud-bearing papillae on the rat tongue. J Comp Neurol. 1991, 304: 172-186. 10.1002/cne.903040203.PubMedGoogle Scholar
- Vogel KS: Development of trophic interactions in the vertebrate peripheral nervous system. Mol Neurobiol. 1993, 7: 363-382.PubMedGoogle Scholar
- Brady R, Zaidi SI, Mayer C, Katz DM: BDNF is a target-derived survival factor for arterial baroreceptor and chemoafferent primary sensory neurons. J Neurosci. 1999, 19: 2131-2142.PubMedGoogle Scholar
- Farinas I, Yoshida CK, Backus C, Reichardt LF: Lack of neurotrophin-3 results in death of spinal sensory neurons and premature differentiation of their precursors. Neuron. 1996, 17: 1065-1078. 10.1016/S0896-6273(00)80240-8.PubMed CentralPubMedGoogle Scholar
- Farinas I, Wilkinson GA, Backus C, Reichardt LF, Patapoutian A: Characterization of neurotrophin and Trk receptor functions in developing sensory ganglia: direct NT-3 activation of TrkB neurons in vivo. Neuron. 1998, 21: 325-334. 10.1016/S0896-6273(00)80542-5.PubMed CentralPubMedGoogle Scholar
- Huang EJ, Wilkinson GA, Farinas I, Backus C, Zang K, Wong SL, Reichardt LF: Expression of Trk receptors in the developing mouse trigeminal ganglion: in vivo evidence for NT-3 activation of TrkA and TrkB in addition to TrkC. Development. 1999, 126: 2191-2203.PubMed CentralPubMedGoogle Scholar
- Lewin GR: Neurotrophins and the specification of neuronal phenotype. Philos Trans R Soc Lond B Biol Sci. 1996, 351: 405-411. 10.1098/rstb.1996.0035.PubMedGoogle Scholar
- Zhou XF, Chie ET, Rush RA: Distribution of brain-derived neurotrophic factor in cranial and spinal ganglia. Exp Neurol. 1998, 149: 237-242. 10.1006/exnr.1997.6716.PubMedGoogle Scholar
- Conover JC, Erickson JT, Katz DM, Bianchi LM, Poueymirou WT, McClain J, Pan L, Helgren M, Ip NY, Boland P, et al: Neuronal deficits, not involving motor neurons, in mice lacking BDNF and/or NT 4. Nature. 1995, 375: 235-238. 10.1038/375235a0.PubMedGoogle Scholar
- Liu X, Ernfors P, Wu H, Jaenisch R: Sensory but not motor neuron deficits in mice lacking NT4 and BDNF. Nature. 1995, 375: 238-241. 10.1038/375238a0.PubMedGoogle Scholar
- Liebl DJ, Tessarollo L, Palko ME, Parada LF: Absence of sensory neurons before target innervation in brain-derived neurotrophic factor-, neurotrophin 3-, and TrkC-deficient embryonic mice. J Neurosci. 1997, 17: 9113-9121.PubMedGoogle Scholar
- Erickson JT, Conover JC, Borday V, Champagnat J, Barbacid M, Yancopoulos G, Katz DM: Mice lacking brain-derived neurotrophic factor exhibit visceral sensory neuron losses distinct from mice lacking NT4 and display a severe developmental deficit in control of breathing. J Neurosci. 1996, 16: 5361-5371.PubMedGoogle Scholar
- Hosley MA, Hughes SE, Morton LL, Oakley B: A sensitive period for the neural induction of taste buds. J Neurosci. 1987, 7: 2075-2080.PubMedGoogle Scholar
- Nagato T, Matsumoto K, Tanioka H, Kodama J, Toh H: Effect of denervation on morphogenesis of the rat fungiform papilla. Acta Anat (Basel). 1995, 153: 301-309.Google Scholar
- Sollars SI: Chorda tympani nerve transection at different developmental ages produces differential effects on taste bud volume and papillae morphology in the rat. J Neurobiol. 2005, 64: 310-320. 10.1002/neu.20140.PubMedGoogle Scholar
- Liebl DJ, Mbiene JP, Parada LF: NT4/5 mutant mice have deficiency in gustatory papillae and taste bud formation. Dev Biol. 1999, 213: 378-389. 10.1006/dbio.1999.9385.PubMedGoogle Scholar
- Mistretta CM, Goosens KA, Farinas I, Reichardt LF: Alterations in size, number, and morphology of gustatory papillae and taste buds in BDNF null mutant mice demonstrate neural dependence of developing taste organs. J Comp Neurol. 1999, 409: 13-24. 10.1002/(SICI)1096-9861(19990621)409:1<13::AID-CNE2>3.0.CO;2-O.PubMed CentralPubMedGoogle Scholar
- Nosrat CA, Blomlof J, ElShamy WM, Ernfors P, Olson L: Lingual deficits in BDNF and NT3 mutant mice leading to gustatory and somatosensory disturbances, respectively. Development. 1997, 124: 1333-1342.PubMedGoogle Scholar
- Sun H, Oakley B: Development of anterior gustatory epithelia in the palate and tongue requires epidermal growth factor receptor. Dev Biol. 2002, 242: 31-43. 10.1006/dbio.2001.0526.PubMedGoogle Scholar
- Zhang C, Brandemihl A, Lau D, Lawton A, Oakley B: BDNF is required for the normal development of taste neurons in vivo. Neuroreport. 1997, 8: 1013-1017. 10.1097/00001756-199703030-00039.PubMedGoogle Scholar
- Nosrat IV, Agerman K, Marinescu A, Ernfors P, Nosrat CA: Lingual deficits in neurotrophin double knockout mice. J Neurocytol. 2004, 33: 607-615. 10.1007/s11068-005-3330-2.PubMedGoogle Scholar
- Nosrat CA, Olson L: Brain-derived neurotrophic factor mRNA is expressed in the developing taste bud-bearing tongue papillae of rat. J Comp Neurol. 1995, 360: 698-704. 10.1002/cne.903600413.PubMedGoogle Scholar
- Nosrat CA, Ebendal T, Olson L: Differential expression of brain-derived neurotrophic factor and neurotrophin 3 mRNA in lingual papillae and taste buds indicates roles in gustatory and somatosensory innervation. J Comp Neurol. 1996, 376: 587-602. 10.1002/(SICI)1096-9861(19961223)376:4<587::AID-CNE7>3.0.CO;2-Y.PubMedGoogle Scholar
- Nosrat CA, Olson L: Changes in neurotrophin-3 messenger RNA expression patterns in the prenatal rat tongue suggest guidance of developing somatosensory nerves to their final targets. Cell Tissue Res. 1998, 292: 619-623. 10.1007/s004410051092.PubMedGoogle Scholar
- Fan L, Girnius S, Oakley B: Support of trigeminal sensory neurons by nonneuronal p75 neurotrophin receptors. Brain Res Dev Brain Res. 2004, 150: 23-39. 10.1016/j.devbrainres.2004.02.008.PubMedGoogle Scholar
- Yee CL, Jones KR, Finger TE: Brain-derived neurotrophic factor is present in adult mouse taste cells with synapses. J Comp Neurol. 2003, 459: 15-24. 10.1002/cne.10589.PubMedGoogle Scholar
- Schecterson LC, Bothwell M: Novel roles for neurotrophins are suggested by BDNF and NT-3 mRNA expression in developing neurons. Neuron. 1992, 9: 449-463. 10.1016/0896-6273(92)90183-E.PubMedGoogle Scholar
- Yan Q, Rosenfeld RD, Matheson CR, Hawkins N, Lopez OT, Bennett L, Welcher AA: Expression of brain-derived neurotrophic factor protein in the adult rat central nervous system. Neuroscience. 1997, 78: 431-448. 10.1016/S0306-4522(96)00613-6.PubMedGoogle Scholar
- Cho TT, Farbman AI: Neurotrophin receptors in the geniculate ganglion. Brain Res Mol Brain Res. 1999, 68: 1-13. 10.1016/S0169-328X(99)00006-6.PubMedGoogle Scholar
- Takeda M, Suzuki Y, Obara N, Tsunekawa H: Immunohistochemical detection of neurotrophin-3 and -4, and their receptors in mouse taste bud cells. Arch Histol Cytol. 2005, 68: 393-403. 10.1679/aohc.68.393.PubMedGoogle Scholar
- Farbman AI, Guagliardo N, Sollars SI, Hill DL: Each sensory nerve arising from the geniculate ganglion expresses a unique fingerprint of neurotrophin and neurotrophin receptor genes. J Neurosci Res. 2004, 78: 659-667. 10.1002/jnr.20297.PubMed CentralPubMedGoogle Scholar
- ElShamy WM, Ernfors P: Brain-derived neurotrophic factor, neurotrophin-3, and neurotrophin-4 complement and cooperate with each other sequentially during visceral neuron development. Journal of Neuroscience. 1997, 17: 8667-8675.PubMedGoogle Scholar
- Patel AV, Krimm RF: BDNF and NT4 regulate geniculate ganglion neuron number at different developmental time points. Society for Neuroscience. 2005, Washington DC. Abstract viewer and Itinerary PlannerGoogle Scholar
- Hellard D, Brosenitsch T, Fritzsch B, Katz DM: Cranial sensory neuron development in the absence of brain-derived neurotrophic factor in BDNF/Bax double null mice. Dev Biol. 2004, 275: 34-43. 10.1016/j.ydbio.2004.07.021.PubMedGoogle Scholar
- Yamout A, Spec A, Cosmano J, Kashyap M, Rochlin MW: Neurotrophic factor receptor expression and in vitro nerve growth of geniculate ganglion neurons that supply divergent nerves. Dev Neurosci. 2005, 27: 288-298. 10.1159/000086708.PubMedGoogle Scholar
- Erickson JT, Brosenitsch TA, Katz DM: Brain-derived neurotrophic factor and glial cell line-derived neurotrophic factor are required simultaneously for survival of dopaminergic primary sensory neurons in vivo. J Neurosci. 2001, 21: 581-589.PubMedGoogle Scholar
- Rochlin MW, O'Connor R, Giger RJ, Verhaagen J, Farbman AI: Comparison of neurotrophin and repellent sensitivities of early embryonic geniculate and trigeminal axons. J Comp Neurol. 2000, 422: 579-593. 10.1002/1096-9861(20000710)422:4<579::AID-CNE7>3.0.CO;2-G.PubMedGoogle Scholar
- Lopez GF, V. PA, Krimm RF: The Regulation of Neural Targeting in the Developing Gustatory System. 2006, Chemical Senses; Sarasota, Florida, 154-Google Scholar
- Rochlin MW, Farbman AI: Trigeminal ganglion axons are repelled by their presumptive targets. J Neurosci. 1998, 18: 6840-6852.PubMedGoogle Scholar
- Mbiene JP, Mistretta CM: Initial innervation of embryonic rat tongue and developing taste papillae: nerves follow distinctive and spatially restricted pathways. Acta Anat (Basel). 1997, 160: 139-158.Google Scholar
- Tessier-Lavigne M, Goodman CS: The molecular biology of axon guidance. Science. 1996, 274: 1123-1133. 10.1126/science.274.5290.1123.PubMedGoogle Scholar
- Dickson BJ: Molecular mechanisms of axon guidance. Science. 2002, 298: 1959-1964. 10.1126/science.1072165.PubMedGoogle Scholar
- Giger RJ, Wolfer DP, De Wit GM, Verhaagen J: Anatomy of rat semaphorin III/collapsin-1 mRNA expression and relationship to developing nerve tracts during neuroembryogenesis. J Comp Neurol. 1996, 375: 378-392. 10.1002/(SICI)1096-9861(19961118)375:3<378::AID-CNE3>3.0.CO;2-#.PubMedGoogle Scholar
- Dillon TE, Saldanha J, Giger R, Verhaagen J, Rochlin MW: Sema3A regulates the timing of target contact by cranial sensory axons. J Comp Neurol. 2004, 470: 13-24. 10.1002/cne.11029.PubMedGoogle Scholar
- Vilbig R, Cosmano J, Giger R, Rochlin MW: Distinct roles for Sema3A, Sema3F, and an unidentified trophic factor in controlling the advance of geniculate axons to gustatory lingual epithelium. J Neurocytol. 2004, 33: 591-606. 10.1007/s11068-005-3329-8.PubMedGoogle Scholar
- Gross JB, Gottlieb AA, Barlow LA: Gustatory neurons derived from epibranchial placodes are attracted to, and trophically supported by, taste bud-bearing endoderm in vitro. Dev Biol. 2003, 264: 467-481. 10.1016/j.ydbio.2003.08.024.PubMedGoogle Scholar
- Lopez GF, Krimm RF: Epithelial overexpression of BDNF and NT4 produces distinct gustatory axon morphologies that disrupt initial targeting. Dev Biol. 2006, 292: 457-468. 10.1016/j.ydbio.2006.01.021.PubMed CentralPubMedGoogle Scholar
- Dontchev VD, Letourneau PC: Nerve growth factor and semaphorin 3A signaling pathways interact in regulating sensory neuronal growth cone motility. J Neurosci. 2002, 22: 6659-6669.PubMedGoogle Scholar
- Paulson RB, Hayes TG, Sucheston ME: Scanning electron microscope study of tongue development in the CD-1 mouse fetus. J Craniofac Genet Dev Biol. 1985, 5: 59-73.PubMedGoogle Scholar
- Miller IJ, Spangler K: Taste bud distribution and innervation on the palate of the rat. Chemical Senses. 1982, 7: 99-108. 10.1093/chemse/7.1.99.Google Scholar
- Zhou Y, Liu HX, Mistretta CM: Bone morphogenetic proteins and noggin: inhibiting and inducing fungiform taste papilla development. Dev Biol. 2006, 297: 198-213. 10.1016/j.ydbio.2006.05.022.PubMedGoogle Scholar
- Hall JM, Hooper JE, Finger TE: Expression of sonic hedgehog, patched, and Gli1 in developing taste papillae of the mouse. J Comp Neurol. 1999, 406: 143-155. 10.1002/(SICI)1096-9861(19990405)406:2<143::AID-CNE1>3.0.CO;2-X.PubMedGoogle Scholar
- Bitgood MJ, McMahon AP: Hedgehog and Bmp genes are coexpressed at many diverse sites of cell-cell interaction in the mouse embryo. Dev Biol. 1995, 172: 126-138. 10.1006/dbio.1995.0010.PubMedGoogle Scholar
- Jung HS, Oropeza V, Thesleff I: Shh, Bmp-2, Bmp-4 and Fgf-8 are associated with initiation and patterning of mouse tongue papillae. Mech Dev. 1999, 81: 179-182. 10.1016/S0925-4773(98)00234-2.PubMedGoogle Scholar
- Okubo T, Pevny LH, Hogan BL: Sox2 is required for development of taste bud sensory cells. Genes Dev. 2006, 20: 2654-2659. 10.1101/gad.1457106.PubMed CentralPubMedGoogle Scholar
- Mistretta CM, Liu HX: Development of fungiform papillae: patterned lingual gustatory organs. Arch Histol Cytol. 2006, 69: 199-208. 10.1679/aohc.69.199.PubMedGoogle Scholar
- Iwatsuki K, Liu HX, Gronder A, Singer MA, Lane TF, Grosschedl R, Mistretta CM, Margolskee RF: Wnt signaling interacts with Shh to regulate taste papilla development. Proc Natl Acad Sci USA. 2007, 104: 2253-2258. 10.1073/pnas.0607399104.PubMed CentralPubMedGoogle Scholar
- Liu F, Thirumangalathu S, Gallant NM, Yang SH, Stoick-Cooper CL, Reddy ST, Andl T, Taketo MM, Dlugosz AA, Moon RT, et al: Wnt-beta-catenin signaling initiates taste papilla development. Nature genetics. 2007, 39: 106-112. 10.1038/ng1932.PubMedGoogle Scholar
- Hall JM, Bell ML, Finger TE: Disruption of sonic hedgehog signaling alters growth and patterning of lingual taste papillae. Dev Biol. 2003, 255: 263-277. 10.1016/S0012-1606(02)00048-9.PubMedGoogle Scholar
- Liu HX, Maccallum DK, Edwards C, Gaffield W, Mistretta CM: Sonic hedgehog exerts distinct, stage-specific effects on tongue and taste papilla development. Dev Biol. 2004, 276: 280-300. 10.1016/j.ydbio.2004.07.042.PubMedGoogle Scholar
- Mistretta CM, Liu HX, Gaffield W, MacCallum DK: Cyclopamine and jervine in embryonic rat tongue cultures demonstrate a role for Shh signaling in taste papilla development and patterning: fungiform papillae double in number and form in novel locations in dorsal lingual epithelium. Dev Biol. 2003, 254: 1-18. 10.1016/S0012-1606(02)00014-3.PubMedGoogle Scholar
- Mistretta CM, Grigaliunas A, Liu HX: Development of gustatory organs and innervating sensory Ganglia. Chem Senses. 2005, 30 (Suppl 1): i52-i53. 10.1093/chemse/bjh109.PubMedGoogle Scholar
- Miller IJ, Preslar AJ: Spatial distribution of rat fungiform papillae. Anat Rec. 1975, 181: 679-684. 10.1002/ar.1091810309.PubMedGoogle Scholar
- Nosrat CA, MacCallum DK, Mistretta CM: Distinctive spatiotemporal expression patterns for neurotrophins develop in gustatory papillae and lingual tissues in embryonic tongue organ cultures. Cell Tissue Res. 2001, 303: 35-45. 10.1007/s004410000271.PubMedGoogle Scholar
- Rochlin MW, Egwiekhor A, Vatterott P, Spec A: BDNF attracts geniculate neurites, NT4 does not. Program No. 501. 5. 2006, Society for Neuroscience; Atlanta, GA, OnlineGoogle Scholar
- Krimm RF, Miller KK, Kitzman PH, Davis BM, Albers KM: Epithelial overexpression of BDNF or NT4 disrupts targeting of taste neurons that innervate the anterior tongue. Dev Biol. 2001, 232: 508-521. 10.1006/dbio.2001.0190.PubMedGoogle Scholar
- Ringstedt T, Ibanez CF, Nosrat CA: Role of brain-derived neurotrophic factor in target invasion in the gustatory system. J Neurosci. 1999, 19: 3507-3518.PubMedGoogle Scholar
- Fritzsch B, Pauley S, Matei V, Katz DM, Xiang M, Tessarollo L: Mutant mice reveal the molecular and cellular basis for specific sensory connections to inner ear epithelia and primary nuclei of the brain. Hearing research. 2005, 206: 52-63. 10.1016/j.heares.2004.11.025.PubMed CentralPubMedGoogle Scholar
- Stone LM, Finger TE, Tam PP, Tan SS: Taste receptor cells arise from local epithelium, not neurogenic ectoderm. Proc Natl Acad Sci USA. 1995, 92: 1916-1920. 10.1073/pnas.92.6.1916.PubMed CentralPubMedGoogle Scholar
- Barlow LA, Northcutt RG: Taste buds develop autonomously from endoderm without induction by cephalic neural crest or paraxial mesoderm. Development. 1997, 124: 949-957.PubMedGoogle Scholar
- Barlow LA, Northcutt RG: Embryonic origin of amphibian taste buds. Dev Biol. 1995, 169: 273-285. 10.1006/dbio.1995.1143.PubMedGoogle Scholar
- Mbiene JP, Roberts JD: Distribution of keratin 8-containing cell clusters in mouse embryonic tongue: evidence for a prepattern for taste bud development. J Comp Neurol. 2003, 457: 111-122. 10.1002/cne.10551.PubMedGoogle Scholar
- Byrne C, Tainsky M, Fuchs E: Programming gene expression in developing epidermis. Development. 1994, 120: 2369-2383.PubMedGoogle Scholar
- Farbman AI: Electron Microscope Study of the Developing Taste Bud in Rat Fungiform Papilla. Dev Biol. 1965, 11: 110-135. 10.1016/0012-1606(65)90040-0.PubMedGoogle Scholar
- Parker MA, Bell ML, Barlow LA: Cell contact-dependent mechanisms specify taste bud pattern during a critical period early in embryonic development. Dev Dyn. 2004, 230: 630-642. 10.1002/dvdy.20086.PubMedGoogle Scholar
- Mbiene JP, Farbman AI: Evidence for stimulus access to taste cells and nerves during development: an electron microscopic study. Microsc Res Tech. 1993, 26: 94-105. 10.1002/jemt.1070260203.PubMedGoogle Scholar
- Mistretta CM: Topographical and histological study of the developing rat tongue, palate and taste buds. Third Symposium on Oral Sensation and Perception: The Mouth of the Infant. Edited by: Bosma JF. 1972, Springfield, IL: Charles C. Thomas, 163-187.Google Scholar
- Delay RJ, Kinnamon JC, Roper SD: Ultrastructure of mouse vallate taste buds: II. Cell types and cell lineage. J Comp Neurol. 1986, 253: 242-252. 10.1002/cne.902530210.PubMedGoogle Scholar
- Yee CL, Yang R, Bottger B, Finger TE, Kinnamon JC: "Type III" cells of rat taste buds: immunohistochemical and ultrastructural studies of neuron-specific enolase, protein gene product 9.5, and serotonin. J Comp Neurol. 2001, 440: 97-108. 10.1002/cne.1372.PubMedGoogle Scholar
- Miura H, Kusakabe Y, Kato H, Miura-Ohnuma J, Tagami M, Ninomiya Y, Hino A: Co-expression pattern of Shh with Prox1 and that of Nkx2.2 with Mash1 in mouse taste bud. Gene Expr Patterns. 2003, 3: 427-430. 10.1016/S1567-133X(03)00081-4.PubMedGoogle Scholar
- Kusakabe Y, Miura H, Hashimoto R, Sugiyama C, Ninomiya Y, Hino A: The neural differentiation gene Mash-1 has a distinct pattern of expression from the taste reception-related genes gustducin and T1R2 in the taste buds. Chem Senses. 2002, 27: 445-451. 10.1093/chemse/27.5.445.PubMedGoogle Scholar
- Seta Y, Seta C, Barlow LA: Notch-associated gene expression in embryonic and adult taste papillae and taste buds suggests a role in taste cell lineage decisions. J Comp Neurol. 2003, 464: 49-61. 10.1002/cne.10787.PubMedGoogle Scholar
- Miura H, Kato H, Kusakabe Y, Tagami M, Miura-Ohnuma J, Ookura T, Shindo Y, Ninomiya Y, Hino A: Shh signaling and regulatory gene expression in mouse taste buds. Chem Senses. 2005, 30 (Suppl 1): i50-i51. 10.1093/chemse/bjh108.PubMedGoogle Scholar
- Suzuki Y, Takeda M, Obara N: Expression of NeuroD in the mouse taste buds. Cell Tissue Res. 2002, 307: 423-428. 10.1007/s00441-001-0504-9.PubMedGoogle Scholar
- Cau E, Casarosa S, Guillemot F: Mash1 and Ngn1 control distinct steps of determination and differentiation in the olfactory sensory neuron lineage. Development. 2002, 129: 1871-1880.PubMedGoogle Scholar
- McLaughlin SK: Erb and c-Kit receptors have distinctive patterns of expression in adult and developing taste papillae and taste buds. J Neurosci. 2000, 20: 5679-5688.PubMedGoogle Scholar
- Zhang LL, Ashwell KW: The development of cranial nerve and visceral afferents to the nucleus of the solitary tract in the rat. Anat Embryol (Berl). 2001, 204: 135-151. 10.1007/s004290100185.Google Scholar
- Sollars SI, Walker BR, Thaw AK, Hill DL: Age-related decrease of the chorda tympani nerve terminal field in the nucleus of the solitary tract is prevented by dietary sodium restriction during development. Neuroscience. 2006, 137: 1229-1236. 10.1016/j.neuroscience.2005.09.040.PubMedGoogle Scholar
- Lasiter PS: Postnatal development of gustatory recipient zones within the nucleus of the solitary tract. Brain Res Bull. 1992, 28: 667-677. 10.1016/0361-9230(92)90245-S.PubMedGoogle Scholar
- Deckwerth TL, Elliott JL, Knudson CM, Johnson EM, Snider WD, Korsmeyer SJ: BAX is required for neuronal death after trophic factor deprivation and during development. Neuron. 1996, 17: 401-411. 10.1016/S0896-6273(00)80173-7.PubMedGoogle Scholar
- Lentz SI, Knudson CM, Korsmeyer SJ, Snider WD: Neurotrophins support the development of diverse sensory axon morphologies. J Neurosci. 1999, 19: 1038-1048.PubMedGoogle Scholar
- White FA, Keller-Peck CR, Knudson CM, Korsmeyer SJ, Snider WD: Widespread elimination of naturally occurring neuronal death in Bax-deficient mice. J Neurosci. 1998, 18: 1428-1439.PubMedGoogle Scholar
- Patel TD, Kramer I, Kucera J, Niederkofler V, Jessell TM, Arber S, Snider WD: Peripheral NT3 signaling is required for ETS protein expression and central patterning of proprioceptive sensory afferents. Neuron. 2003, 38: 403-416. 10.1016/S0896-6273(03)00261-7.PubMedGoogle Scholar
- Patel TD, Jackman A, Rice FL, Kucera J, Snider WD: Development of sensory neurons in the absence of NGF/TrkA signaling in vivo. Neuron. 2000, 25: 345-357. 10.1016/S0896-6273(00)80899-5.PubMedGoogle Scholar
- Krimm RF, Hill DL: Early prenatal critical period for chorda tympani nerve terminal field development. J Comp Neurol. 1997, 378: 254-264. 10.1002/(SICI)1096-9861(19970210)378:2<254::AID-CNE8>3.0.CO;2-2.PubMedGoogle Scholar
- Mangold JE, Hill DL: Extensive reorganization of primary afferent projections into the gustatory brainstem induced by feeding a sodium-restricted diet during development: less is more. J Neurosci. 2007, 27: 4650-4662. 10.1523/JNEUROSCI.4518-06.2007.PubMed CentralPubMedGoogle Scholar
- Pattyn A, Morin X, Cremer H, Goridis C, Brunet JF: Expression and interactions of the two closely related homeobox genes Phox2a and Phox2b during neurogenesis. Development. 1997, 124: 4065-4075.PubMedGoogle Scholar
- Qian Y, Fritzsch B, Shirasawa S, Chen CL, Choi Y, Ma Q: Formation of brainstem (nor)adrenergic centers and first-order relay visceral sensory neurons is dependent on homeodomain protein Rnx/Tlx 3. Genes Dev. 2001, 15: 2533-2545. 10.1101/gad.921501.PubMed CentralPubMedGoogle Scholar
- Cheng L, Arata A, Mizuguchi R, Qian Y, Karunaratne A, Gray PA, Arata S, Shirasawa S, Bouchard M, Luo P, et al: Tlx3 and Tlx1 are post-mitotic selector genes determining glutamatergic over GABAergic cell fates. Nat Neurosci. 2004, 7: 510-517. 10.1038/nn1221.PubMedGoogle Scholar
- Qian Y, Shirasawa S, Chen CL, Cheng L, Ma Q: Proper development of relay somatic sensory neurons and D2/D4 interneurons requires homeobox genes Rnx/Tlx-3 and Tlx-1. Genes Dev. 2002, 16: 1220-1233. 10.1101/gad.982802.PubMed CentralPubMedGoogle Scholar
- Gaultier C: Genes and genetics in respiratory control. Paediatric respiratory reviews. 2004, 5: 166-172. 10.1016/j.prrv.2004.04.012.PubMedGoogle Scholar
- Katz DM: Regulation of respiratory neuron development by neurotrophic and transcriptional signaling mechanisms. Respiratory physiology & neurobiology. 2005, 149: 99-109. 10.1016/j.resp.2005.02.007.Google Scholar
- Ramanantsoa N, Vaubourg V, Dauger S, Matrot B, Vardon G, Chettouh Z, Gaultier C, Goridis C, Gallego J: Ventilatory response to hyperoxia in newborn mice heterozygous for the transcription factor Phox2b. American journal of physiology. 2006, 290: R1691-1696.PubMedGoogle Scholar
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