Skip to main content

Table 1 Relative percentage of VGLUT1 and VGAT mRNA levels compared to β-actin expression.

From: The role of the t-SNARE SNAP-25 in action potential-dependent calcium signaling and expression in GABAergic and glutamatergic neurons

Fluorescence-activated cell sorting

Sample

GFP Phenotype

VGLUT1

VGAT

Immature cortical cells

Negative

2.37%

n. d.*

 

Positive

n. d.

7.89%

Adult cortical cells

Negative

3.91%

n. d.

 

Positive

n. d.

9.51%

Laser capture microdissection

Region

GFP Phenotype

VGLUT1

VGAT

Reticular Nucleus

Positive

n. d.

12.68%

Caudate

Positive

n. d.

14.07%

Purkinje cells

Positive

n. d.

15.39%

Hippocampal interneurons (CA1)

Positive

n. d.

12.45%

Hippocampal pyramidal cells (CA1)

Negative

11.95%

n. d.

Cerebellar granule cells

Negative

13.56%

n. d.

  1. * n. d. (not detected) is considered less than 0.001% (i.e. Ct value > 10 cycles) of gene expression in comparable amounts of whole brain samples (see Materials and Methods).