Figure 4
Simultaneous presentation of 15°C and isoamyl alcohol to 15°C-cultivated worms at 20°C room temperature. (A and B) Indices of isoamyl alcohol-single presentation (open circle), 15°C-single presentation (open triangle) and simultaneous presentation (closed square). Gray line indicates the sum of the chemotaxis index for isoamyl alcohol and the thermotaxis index for 15°C in single presentations. Each data point is presented as the mean ± SEM. Asterisks indicate significant difference (p < 0.05) between simultaneous presentation index (solid line marked with closed square) and the summed index (gray line). Time courses of indices for isoamyl alcohol when 1/10,000 dilution was used (A) and when 1/1,000 dilution was used (B). (C and D) FRn every 10 min during simultaneous presentation assay. (E) Track of a single worm under the same conditions as (B). (F) Distribution of tracks of single worms (n = 20). Each track of single worm was recorded for 90 min. High accumulation is indicated by red, and low accumulation is indicated by blue.