Skip to main content
Figure 4 | BMC Neuroscience

Figure 4

From: TRPM5-expressing microvillous cells in the main olfactory epithelium

Figure 4

Negative immunoreactivity for CNGA2, OMP, and a neuronal marker PGP 9.5 in the TRPM5 (GFP)-expressing microvillous cells. Sections were immunoreacted to antibodies against the CNGA2 (A-C), OMP (D-F), and PGP 9.5 (G-L) respectively. A, D, and G. Fluorescence images showing GFP-expressing microvillous cells in the OE. The microvillous cells in (A) are marked with asterisks to distinguish from the GFP-expressing OSNs. B, E, and H. Fluorescence images of immunoreaction to antibodies against the CNGA2 (B), OMP (E) and PGP 9.5 (H) respectively. All three antibodies labeled OSNs, However, these antibodies failed to label any TRPM5 (GFP)-expressing microvillous cells. C, F, and I. Overlaid images showing there was no co-localization of GFP signal and OMP- or CNGA2- or PGP 9.5 immunoreactivity in microvillous cells. Note in (C) where both GFP-positive OSNs and microvillous cells are present, GFP-expressing OSNs showed co-localization of CNGA2 immunoreaction. J, K, and L. Enlarged images of G-L showing the anti-PGP9.5 antibody did not label a GFP-positive microvillous cell. There were also apparently no PGP9.5-labeled nerve fibers that closely associated to the cell. Scales: A-F, 10 μm; G-I, 20 μm; J-L, 5 μm.

Back to article page