Figure 6

Immunolabeling of trigeminal peptidergic fibers in the OE. A. The antibody against the peptide CGRP labeled intraepithelial trigeminal fibers in the OE, Some fibers reached to the superficial layer where the TRPM5-expressing microvillous cells were located but was found adjacent only to a small subset of cells, and did not wrap tightly around the cell bodies as seen for TRPM5-expressing solitary chemoreceptor cells of the anterior nasal epithelium[33]. B. Image from an epithelial strip reacted with the antibody against substance P. Most of the substance P positive fibers ran at the base of the epithelium, with some intraepithelial fibers. Note that most of the GFP expressing microvillous cells did not associate closely with the fibers. Scales: 10 μm.