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Figure 1 | BMC Neuroscience

Figure 1

From: CLOCK expression identifies developing circadian oscillator neurons in the brains of Drosophila embryos

Figure 1

DAC is co-expressed with CLK in the CNS of L3 larvae. L3 larval brains were dissected and fixed at ZT1, immunostained with CLK and DAC antibodies, and imaged by confocal microscopy. (A-C) Projected Z-series images of the CNS, where dorsal is at the top. (A) DAC expression in the CNS. Arrows denote DAC immunoreactivity in Kenyon cells (KC) and the optic lobe (OL). (B) CLK expression in the same brain as panel A. Arrows denote CLK immunoreactivity in KCs and the OL. (C) Superimposed dual laser image of DAC and CLK immunostaining in the same larval CNS as panels A and B. Co-localization of DAC (green) and CLK (red) is seen as yellow. Arrowheads, CLK positive/DAC negative cells. (D-F) Magnified 1 μm optical section through the left hemisphere of an L3 larval brain. OL, optic lobe. (D) DAC immunostaining. (E) CLK immunostaining. (F) Superimposed dual laser image of DAC and CLK immunostaining. Arrowheads, CLK positive/DAC negative cells. Images are representative of three independent experiments. Six or more larval CNSs were examined in each experiment. Z-series images are projections over 32 optical sections at 2.5 μm per optical section.

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