Figure 4
Surface markers expressions of radio-resistant cells derived from human glioblastoma cells. The cultured radio-resistant cells (1 × 106) were stained with CD133, CD117, CD45, and CD71 coupled to fluorescein isothiocyanate (FITC) or PE (phycoerythrin) at various culture passages after gamma-irradiation, and then analyzed via flow cytometry. (A) Each cell subpopulation rate of A172 cells after gamma-irradiation. (B) Representative flow cytometry histogram of radio-resistant A172 subpopulations for CD133, CD117, CD45 and CD71 at three culture passages after gamma-irradiation. (C) Each cell subpopulation rate of GBM2 cells after gamma-radiation. (D) Representative flow cytometry histogram of radio-resistant GBM2 subpopulations for CD133, CD117, CD45 and CD71 at three culture passages after gamma-radiation. The results of triplicate samples were expressed as the means ± SD. **p < 0.01 vs control.