Figure 5From: Alterations in the Notch4 pathway in cerebral endothelial cells by the HIV aspartyl protease inhibitor, nelfinavirHIV PIs do not affect APP expression in CEC. A) Western analyses of the expression of total APP after 5 μM exposure to A) saquinavir (SQV), indinavir (INV), nelfinavir (NFV), and ritonavir (RTV) for various lengths of time (1, 24, 48 and 96 h). B) quantitative real-time PCR analyses of APP mRNA levels after exposure to saquinavir (S), indinavir (I), nelfinavir (N), or ritonavir (R) for 1 or 48 h. No statistically significant differences were calculated by one way ANOVA. Graphs reflect results from three or more separate experiments.Back to article page