Figure 6

HIV PIs affect Notch 4 protein expression and processing in CEC. Western analyses of the expression of total Notch 4 (dark bars) (*p ≤ 0.002) and the percentage of Notch 4 processing (NICD) (lighter bars) after 5 μM exposure to A) saquinavir (SQV), B) indinavir (INV), C) nelfinavir (NFV) (*p ≤ 0.007), and D) ritonavir (RTV) (*p ≤ 0.001) for various lengths of time (1, 24, 48 and 96 h). E) quantitative real-time PCR analyses of Notch4 mRNA levels after exposure to saquinavir (S), indinavir (I), nelfinavir (N), or ritonavir (R) for 1 or 48 h. *p ≤ 0.001 by one way ANOVA with Dunnett's post hoc test when compared to untreated control. Graphs reflect results from three or more separate experiments.