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Table 1 Composition of culture media

From: Evidence for neuroprotective properties of human umbilical cord blood cells after neuronal hypoxia in vitro

   

Supplements in DMEM

Day of culture

Culture condition

Description of media

FCS (15%)

RA (10 μM)

BDNF (5 ng/ml)

HSA (0.1%)

Ham's F12

Seeding

Normoxia

Maintenance medium (MM)

x

-

-

-

-

1 – 4

Normoxia

Basic medium (BM)

x

x

-

-

-

5 – 16

Normoxia

Differentiation medium (DM)

-

x

x

x

x

16 – 18

Normoxia/hypoxia

Differentiation medium (DM)

-

x

x

x

x

18 – 21

Normoxia

Post-hypoxic medium (PHM)

-

-

x

x

x

  1. The experimental design demanded varying culture media. Supplements in DMEM were purchased from: fetal calf serum (FCS, PAN-Biotech, Aidenbach, Germany), all-trans-retinoic acid (RA, Sigma-Aldrich, Steinheim, Germany), recombinant brain-derived neurotrophic factor (rhBDNF; ImmunoTools, Friesoythe, Germany), human serum albumin (HSA, PAN Biotech, Aidenbach, Germany).
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BMC Neuroscience

ISSN: 1471-2202

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