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Figure 5 | BMC Neuroscience

Figure 5

From: Differential production of superoxide by neuronal mitochondria

Figure 5

Immunoblotting of Mitochondrial Components. Isolated mitochondrial samples standardized for protein content were compared to corresponding mitochondria depleted samples for the presence of the mitochondrial membrane proteins Complexes I-IV, and VDAC. Standardized mitochondrial preparations were also investigated for the peroxisomal marker PMP70. Samples were subject to an electrophoresis and were transferred overnight to nitrocellulose. Samples were incubated with a mouse a primary antibody corresponding the protein of interest, and subsequently treated with an HRP-conjugated antibody. Each mitochondrial protein shows that there is considerable purification of mitochondria compared to the mitochondria depleted samples for cerebral, neuroblastoma, and undifferentiated RGC-5 cells. There is a nearly 18-fold increase in the expression of complex I in cerebral cells compared to RGC-5. Similar levels of expression in PMP70 and complex II were seen in all three samples.

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