Figure 5From: Differential production of superoxide by neuronal mitochondriaImmunoblotting of Mitochondrial Components. Isolated mitochondrial samples standardized for protein content were compared to corresponding mitochondria depleted samples for the presence of the mitochondrial membrane proteins Complexes I-IV, and VDAC. Standardized mitochondrial preparations were also investigated for the peroxisomal marker PMP70. Samples were subject to an electrophoresis and were transferred overnight to nitrocellulose. Samples were incubated with a mouse a primary antibody corresponding the protein of interest, and subsequently treated with an HRP-conjugated antibody. Each mitochondrial protein shows that there is considerable purification of mitochondria compared to the mitochondria depleted samples for cerebral, neuroblastoma, and undifferentiated RGC-5 cells. There is a nearly 18-fold increase in the expression of complex I in cerebral cells compared to RGC-5. Similar levels of expression in PMP70 and complex II were seen in all three samples.Back to article page