| Cerebral | Neuroblastoma | RGC-5 |
---|
 | N = 7 | N = 7 | N = 8 |
---|
 | Mean | SEM | Mean | SEM | Mean | SEM |
---|
Mitochondria
| 0.208 | 0.033 | 0.318 | 0.035 | 0.030 | 0.004 |
Glutamate/Malate
| 0.262 | 0.028 | 0.560 | 0.054 | 0.046 | 0.004 |
Rotenone
| 0.279 | 0.035 | 0.383 | 0.050 | 0.048 | 0.004 |
- The rates of superoxide production were determined after the addition of the complex I substrates glutamate (10 mM) and malate (5 mM) and the complex I inhibitor rotenone (6.7 μM). Superoxide was indirectly measured using the H2O2 probe Amplex Red in the presence of horseradish peroxidase (HRP). The values are given in nmol H2O2/min/mg protein ± standard error of the mean (SEM), with n being the number of independent samples for each mitochondria type.