| Cerebral | Neuroblastoma | RGC-5 |
---|
 | N = 7 | N = 7 | N = 8 |
---|
 | Mean | SEM | Mean | SEM | Mean | SEM |
---|
Mitochondria
| 0.193 | 0.027 | 0.348 | 0.042 | 0.028 | 0.005 |
Succinate
| 0.216 | 0.022 | 0.307 | 0.024 | 0.031 | 0.005 |
Antimycin A
| 0.540 | 0.116 | 0.277 | 0.034 | 0.024 | 0.004 |
- The rates of superoxide production were determined after the addition of the complex II substrate succinate (10 mM) and the complex III inhibitor antimycin A (0.5 μM). Superoxide was indirectly measured using the H2O2 probe Amplex Red in the presence of horseradish peroxidase (HRP). The values are given in nmol H2O2/min/mg protein ± standard error of the mean (SEM), with n being the number of independent samples for each mitochondria type.