| Undifferentiated RGC-5 | Differentiated RGC-5 |
---|
 | N = 8 | N = 2 |
---|
 | Mean | SEM | Mean | SEM |
---|
Mitochondria
| 0.030 | 0.004 | 0.033 | 0.004 |
Glutamate/Malate
| 0.046 | 0.004 | 0.052 | 0.003 |
Rotenone
| 0.048 | 0.004 | 0.055 | 0.003 |
- The rates of superoxide production were determined after the addition of the complex I substrates glutamate (10 mM) and malate (5 mM) and the complex I inhibitor rotenone (6.7 μM). Superoxide was indirectly measured using the H2O2 probe Amplex Red in the presence of horseradish peroxidase (HRP). The values are given in nmol H2O2/min/mg protein ± standard error of the mean (SEM), with n being the number of independent samples for each mitochondria type.