Table 7 Primers Sequences used for RT-PCR Analysis and Expected Product Sizes
From: Differential production of superoxide by neuronal mitochondria
Gene | Subunit | Primer Sequence 5'-3' | PCR Product |
|---|---|---|---|
SOD2 | - | F: ATT AAC GCG CAG ATC ATG CA | 89 bp |
| Â | Â | R: TCG TGG TAC TTC TCC TCG GTG A | Â |
VDAC | - | F: ATG CCT GCT TTT CGG CCA A | 118 bp |
| Â | Â | R: GCT GGA TGG CAA GAA CGT CAA | Â |
PMP70 | - | F: CTC GGC CTG CAC GGT AAG | 78 bp |
| Â | Â | R: CAC AGC TCG CTC TTT CTT TCC T | Â |
C I | NDUFA9 | F: TTG TCA ACC ATC TTG GAC GAA T | 166 bp |
| Â | Â | R: TTG CTG TGC TGC ACT GCT TTC | Â |
C II | SDHA | F: GAG GAA GCA CAC CCT CTC ATA | 101 bp |
| Â | Â | R: TAG CAC AGT CAG CCT CAT TCA A | Â |
C III | UQCRC2 | F: AAA TTG ACG CTG TGG CTG ATG | 101 bp |
| Â | Â | R: AGG CGT ATG TCC CAA GTT TCC | Â |
C IV | MTCO1 | R: AGC CCC TGA TAT AGC ATT CCC | 116 bp |
| Â | Â | F: TTC ATC CTG TTC CAG CTC CAG | Â |
C V | ATP5A1 | F: CTT ATC CAA GCA GGC TGT TGC | 134 bp |
| Â | Â | R: AAT CGT TCA TCT TGG CTG CTC | Â |