Figure 10

Immunolocalization of synapsin in the olfactory lobes shows that synaptic neuropil is confined to the olfactory glomeruli and the non-columnar olfactory neuropil (letters B, D, F). A1, A2: two optical sections (Apotome structured illumination technique) from different levels of one vibratome section. The non-columnar olfactory neuropils B and F are merged here and line the dorsal part of the median foramen. The non-columnar olfactory neuropil D is embedded within the olfactory glomeruli. The boxed area in A2 is shown in a higher magnification in C. This optical section (Apotome structured illumination technique) shows that there is not any overlap between the synaptic regions of both types of olfactory neuropils (columnar versus non-columnar). D shows a surface reconstructions obtained from a z-series of confocal images that were directly loaded into Amira and processed for semiautomatic segmentation using Amira's "wrap" module.