Figure 1
From: The function of Shp2 tyrosine phosphatase in the dispersal of acetylcholine receptor clusters
Shp2 expression in embryonic Xenopus muscle. In situ hybridization assays for Shp2 mRNA were carried out with anti-sense (A) and control sense (B) probes using stage 22 (A and B, upper) and 28 (lower) Xenopus embryos. Specific labeling was observed with Shp2 mRNA anti-sense probe in myotomal muscle and brain. C. TX-100 total protein extracts of Xenopus tadpole tail muscle ("X") and cultured C2 myotubes ("C") were immunoblotted with an anti-Shp2 monoclonal antibody. A band of about 70 kD was observed in both samples. Positions of molecular weight markers are indicated on the left.