Figure 7
Immunohistochemical verification of selected genes. (A) Consistent with microarray (chip) results, GFAP (green fluorescence) showed signal enhancement in astrocytes of cerebrum in 12 and 18 wk PS-NA mice. CD68 (black) microglia/macrophage stains showed sparse positive cells in cerebrum of WT and 4 wk PS-NA mice. CD68 signal increases were evident in 12 and 18 wks PS-NA mice. Caspase 3 (Casp3, green fluorescence) signals stained Purkinje cells (arrows) in PS-NA 12 wk and older mice. DAPI (blue fluorescence) stains nuclei. (B) Microarray (chip) results of expression changes for GFAP, CD68 and Caspase 3. The dotted line represents 1.5 fold. Time points for PS-NA and 4L/PS-NA are 0 day (white columns), 4 wk (line columns), 12 wk (black columns) and 18 wk (hatched columns); for PS-/- are 0 (white columns), 10 (line columns), 20 (black columns) and 25 (hatched columns) days.