Figure 5
From: Defects in the retina of Niemann-pick type C 1 mutant mice
Defects in amacrine cells in the Npc1 -/-retina. A-F: Retinal sections from P65 Npc1+/+(A-C) and Npc1-/-(D-F) mice were immunostained with an antibody against tyrosine hydroxylase (TH, red). Nuclei were stained with DAPI (blue). B, E show magnification of the INL of the retina from A, D, and C, F of the IPL from A, D, respectively. G-L: Double immunostaining with antibodies against calbindin (red in G-L) and TH (green in G-L) revealed ectopically extended neurites of the Npc1-/- amacrine cells (arrows in J, K; yellow in K). Abbreviations: ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer; S1-S5, five parallel sublaminae of INL. Scale bars: 10 μm in B for B, C, E, F, H, K, and in I for I, L; 50 μm in A for A, D, G, J.