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Figure 9 | BMC Neuroscience

Figure 9

From: Defects in the retina of Niemann-pick type C 1 mutant mice

Figure 9

Transmission electron microscopy of the optic nerve in Npc1 +/+(A) and Npc1 -/-(B-F) animals. A: Regular bundle arrangement in the Npc1+/+ optic nerve. Two astrocytes with primary processes enwrapping numerous myelinated optic axons are labelled green. B: Individual fiber bundles of optic nerve filled with inclusion material in the Npc1-/- mouse (yellow). Arrows point at two examples of disturbed myelination pattern. C: An axon (yellow-colored) with regular arrangement of neurofilaments and one probably irregular myelin-like inclusion body is clearly demarcated by its cell membrane and even indented by the autophagic material of the adjacent oligodendrocyte (weak red). In contrast to Figure 9D, the immediate surrounding cell is loaded with autophagic material and enwraps the axon completely. D: The axon is filled with inclusion material, whereas the myelin sheath remained intact (yellow). E: Large glia bundles (green), some of which contain inclusions in the Npc1-/- mouse; yellow color shows the nerve fibers bundles with myelin-like material (arrows). Elongated gap junctions appear morphologically intact (arrowhead). F: Magnification of the area outlined by a rectangle in E; Two adjacent astrocytes are connected with apparently intact gap junctions (arrows). Scale bar: 500 nm in C.

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