Figure 1

Reelin labeling at the light microscope. A) Reelin immunolabeling in superficial and mid-layers of the cortex. As expected, reelin-labeled neurons are present across different layers of the cortex, and diffuse labeling is mostly present in the superficial layers. B) High magnification of the area indicated with a dashed-line box in A. This image shows a reelin-immunolabeled blood capillary in layer I of the cortex (empty black arrows). C) Reelin labeling in deeper layers of the cortex. Scattered reelin-labeled neurons in these deeper layers present moderate labeling compared to superficial layers. D) High magnification image of the area indicated with a box in C. The capillary is negative for reelin labeling (empty white arrows). E) Reelin labeling in the lateral part of the rostral hippocampus. Note the row of lightly reelin-labeled neurons in the CA1, as well as the diffuse reelin immunolabeling in the stratum lacunosum moleculare (slm). F) High magnification image of an area neighboring the slm, corresponding to the dashed-line area indicated in E. Note the intense reelin labeling surrounding this capillary (empty black arrow). In addition there is a strongly labeled neuron in close proximity (black arrowhead). G) Reelin labeling in the medial part of the rostral hippocampus. The corpus callosum (cc) appears dorsally bordering the hippocampus. H) High magnification image of the dashed-line box area in G. Note the unlabeled capillary (empty white arrow) located in close proximity to a strongly labeled neuron (black arrowhead). cc: corpus callosum; CA1: Cornu Ammonis layer I; slm: stratum lacunosum moleculare. Scale bars: 10 microns in A, C, G, E; 100 microns in B, D, F, H.