Figure 4

Cellular localisation of hepcidin protein in rat brain in neurons, astrocytes and endothelial cells. Hepcidin protein was visible in the molecular layer and glomerular cells of the olfactory bulb (A), dentate gyrus granule cells (B), white matter tracts of corpus callosum, cortex and striatum (C). Hepcidin was present in the upper layers of the cortex but limited expression was observed in β3 tubulin-positive neurons (D-F). Hepcidin expression was seen in the cortex mainly in astrocytes and CA region of the hippocampus with co-localisation noted in GFAP positive cells (G-I). Hepcidin was present in the ependymal cells of lateral ventricle (arrow) and cells within the subventricular zone where co-localisation with GFAP in the wall of the ventricle was observed (J-L). Protein expression and co-localisation was indicated with arrow in relevant panels. The scale bar in J represents 50 μm in A to C, J and K; 100 μm in D-I; 25 μm in B and L.