Table 1 List of the primary antibodies used in this study
From: Expression and cellular localization of hepcidin mRNA and protein in normal rat brain
Antibody | Species | Dilution | Supplier/cat.number |
|---|---|---|---|
Anti-hepcidin | Rabbit (polyclonal) | 1: 200 for IHC | Abcam (Ab30760) |
1:100 for WB | |||
Anti-ferroportin | Mouse (monoclonal) | 1: 1000 for IHC | Abcam (ab93438) |
Anti-GFAP | Mouse (monoclonal) | 1:1000 for IHC | Sigma (G3893 Clone G-A-5) |
Anti-βIII-tubulin | Mouse (monoclonal) | 1:1000 for IHC | Millipore (clone 2G10, neuronal | 05–559) |
Anti β-actin | Mouse (monoclonal) | 1:10000 for WB | Sigma (clone AC-74, A5316) |
Anti-CD31 | Mouse (monoclonal) | 1: 1000 for IHC | Abcam (ab9498) |
Anti-PDGFβR1 | Mouse (monoclonal) | 1: 1000 for IHC | Abcam (ab69506) |
Anti-NG2 | Mouse (monoclonal) | 1: 1000 for IHC | Abcam (ab50009) |
Anti-S100β | Mouse (monoclonal) | 1: 1000 for IHC | Abcam (ab4066) |
Anti-VEGF | Mouse (monoclonal) | 1: 1000 for IHC | Abcam (ab46154) |
Anti-albumin | Mouse (monoclonal) | 1:1000 for WB | Abcam (ab92469) |
Anti-β2Microglobulin | Mouse (monoclonal) | 1:1000 for WB | Abcam (759) |