Figure 5From: Shox2 is required for the proper development of the facial motor nucleus and the establishment of the facial nervesLoss of Shox2 in the brain results in subtle changes in the expression pattern of Isl1 and Phox2b in the E14.5 facial motor nucleus. a Diagram of a sagittal section through the E14.5 brain highlights the regions represented in b–r. b X-gal stained sagittal sections through E14.5 embryos shows decreased staining in the facial motor nucleus (nVII) of Nestin-Cre; Shox2 lacZ/flox (B″) embryos that lack Shox2 function, as compared to controls (B′). c–r ISH on E14.5 control (c, e, g, i, k, m, o, q) and Nestin-Cre; Shox2 flox/− (d, f, h, j, l, n, p, r) sagittal sections shows loss of Shox2 expression (compare c to d, dashed-circle), and subtle changes in the spatial expression patterns of Isl1 (compare e to f, arrow), Phox2b (compare g to h, arrow), Cntn2 (compare i to j, dashed-circle), Tubb3 (compare k to l, dashed-circle), Periph (compare m to n, arrow), Slc18a3 (compare o to p, arrow) and Slit2 (compare q to r, arrow) in the developing facial motor nucleus. The dashed-circles in paired panels being compared (e.g. B′ and B″) are the same size, and this applies from B′ to r. Scale bar 250 μm.Back to article page